Prenatal repair of myelomeningocele with aligned nanofibrous scaffolds - A pilot study in sheep

Payam Saadai, Yvette S. Nout, Jose Encinas, Aijun Wang, Timothy L. Downing, Michael S. Beattie, Jacqueline C. Bresnahan, Song Li, Diana L Farmer

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background/Purpose: Spinal cord damage in myelomeningocele (MMC) results from abnormal cord development and subsequent local trauma. Prenatal surgery prevents additional neural injury. However, existing damage is not reversed. Biodegradable nanofibrous scaffolds (NSs) promote regeneration of neural tissues. They mimic the microtopography of the extracellular matrix and guide tissue formation and organization. The purpose of this pilot study was to evaluate the practicality and safety of using biodegradable NS as a regenerative device in prenatal MMC repair. Methods: Two fetal lambs underwent a surgically induced MMC defect followed by open fetal repair using aligned biodegradable NS. Lambs were killed at day 138. Spinal cords were examined for inflammation or fibrosis and stained for spinal cord architecture, myelin, and neuron cell bodies. Results: Prenatal repair with NS demonstrated technical feasibility. There was no evidence of a surrounding inflammatory response or foreign-body reaction to the scaffold. Conclusion: Biodegradable NS can be used surgically for the prenatal repair of MMC in a large animal model and does not appear to elicit an inflammatory or fibrotic reaction in fetal tissue. Further studies will determine their potential for neural cell infiltration, delivery of growth factors, drugs or stem cells, and functional recovery greater than standard repair.

Original languageEnglish (US)
Pages (from-to)2279-2283
Number of pages5
JournalJournal of Pediatric Surgery
Volume46
Issue number12
DOIs
StatePublished - Dec 2011

Fingerprint

Meningomyelocele
Sheep
Spinal Cord
Foreign-Body Reaction
Wounds and Injuries
Myelin Sheath
Extracellular Matrix
Regeneration
Intercellular Signaling Peptides and Proteins
Fetus
Fibrosis
Stem Cells
Animal Models
Inflammation
Safety
Neurons
Equipment and Supplies
Pharmaceutical Preparations

Keywords

  • Biomaterials
  • Fetal surgery
  • Myelomeningocele
  • Nanotechnology
  • Scaffold
  • Spina bifida

ASJC Scopus subject areas

  • Surgery
  • Pediatrics, Perinatology, and Child Health

Cite this

Prenatal repair of myelomeningocele with aligned nanofibrous scaffolds - A pilot study in sheep. / Saadai, Payam; Nout, Yvette S.; Encinas, Jose; Wang, Aijun; Downing, Timothy L.; Beattie, Michael S.; Bresnahan, Jacqueline C.; Li, Song; Farmer, Diana L.

In: Journal of Pediatric Surgery, Vol. 46, No. 12, 12.2011, p. 2279-2283.

Research output: Contribution to journalArticle

Saadai, Payam ; Nout, Yvette S. ; Encinas, Jose ; Wang, Aijun ; Downing, Timothy L. ; Beattie, Michael S. ; Bresnahan, Jacqueline C. ; Li, Song ; Farmer, Diana L. / Prenatal repair of myelomeningocele with aligned nanofibrous scaffolds - A pilot study in sheep. In: Journal of Pediatric Surgery. 2011 ; Vol. 46, No. 12. pp. 2279-2283.
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