Preliminary studies on bovine embryo survival following short-term storage at 4°C

Robert Bondurant, C. B. Anderson, M. P. Boland, P. T. Cupps, M. A. Hughes

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A total of 113 non-surgically collected bovine embryos, 5-8 days of age, were stored for 48 hours at 4°C in a modified phosphate-buffered saline solution (PBS). Following storage, embryos were cultured for 8-12 hours at 37°C, and those which were morphologically normal were transferred to synchronized recipients by several methods designed to achieve twin pregnancies. Embryos which were collected and transferred on the same day served as controls. Of 113 embryos stored, 47 (42%) appeared to be transferable after the brief culture period. There was a marked breed effect on viability after refrigeration, with Hereford embryos surviving significantly better than Angus embryos (71% vs. 12%, respectively, p < .001). Post-transfer embryo survival of stored and control embryos, based on actual calvings, was 34 and 48 percent, respectively, a difference which was not significant (p=0.3). A marked difference in pregnancy rate following non-surgical transfer by 2 different technicians was noted (50% vs. 21.7%, respectively).

Original languageEnglish (US)
Pages (from-to)223-230
Number of pages8
JournalTheriogenology
Volume17
Issue number2
DOIs
StatePublished - Jan 1 1982
Externally publishedYes

Fingerprint

embryo (animal)
Embryonic Structures
cattle
Refrigeration
Twin Pregnancy
technicians
Embryo Transfer
Hereford
embryo transfer
Pregnancy Rate
Angus
refrigeration
pregnancy rate
Sodium Chloride
sodium chloride
Phosphates
viability
pregnancy
phosphates
breeds

ASJC Scopus subject areas

  • Small Animals
  • Food Animals
  • Animal Science and Zoology
  • Equine

Cite this

Preliminary studies on bovine embryo survival following short-term storage at 4°C. / Bondurant, Robert; Anderson, C. B.; Boland, M. P.; Cupps, P. T.; Hughes, M. A.

In: Theriogenology, Vol. 17, No. 2, 01.01.1982, p. 223-230.

Research output: Contribution to journalArticle

Bondurant, Robert ; Anderson, C. B. ; Boland, M. P. ; Cupps, P. T. ; Hughes, M. A. / Preliminary studies on bovine embryo survival following short-term storage at 4°C. In: Theriogenology. 1982 ; Vol. 17, No. 2. pp. 223-230.
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