Preliminary studies on bovine embryo survival following short-term storage at 4°C

Robert Bondurant, C. B. Anderson, M. P. Boland, P. T. Cupps, M. A. Hughes

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


A total of 113 non-surgically collected bovine embryos, 5-8 days of age, were stored for 48 hours at 4°C in a modified phosphate-buffered saline solution (PBS). Following storage, embryos were cultured for 8-12 hours at 37°C, and those which were morphologically normal were transferred to synchronized recipients by several methods designed to achieve twin pregnancies. Embryos which were collected and transferred on the same day served as controls. Of 113 embryos stored, 47 (42%) appeared to be transferable after the brief culture period. There was a marked breed effect on viability after refrigeration, with Hereford embryos surviving significantly better than Angus embryos (71% vs. 12%, respectively, p < .001). Post-transfer embryo survival of stored and control embryos, based on actual calvings, was 34 and 48 percent, respectively, a difference which was not significant (p=0.3). A marked difference in pregnancy rate following non-surgical transfer by 2 different technicians was noted (50% vs. 21.7%, respectively).

Original languageEnglish (US)
Pages (from-to)223-230
Number of pages8
Issue number2
StatePublished - Jan 1 1982
Externally publishedYes

ASJC Scopus subject areas

  • Small Animals
  • Food Animals
  • Animal Science and Zoology
  • Equine


Dive into the research topics of 'Preliminary studies on bovine embryo survival following short-term storage at 4°C'. Together they form a unique fingerprint.

Cite this