Preliminary observations on reproduction in a female sheep-goat chimaera.

G. B. Anderson, N. A. Ruffing, Robert Bondurant, R. L. Pashen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Four oestrous cycles of a female sheep-goat chimaera were monitored by using a vasectomised ram. The mean (+/- se) length of the cycle was 18.5 +/- 0.64 days with a range from 17 to 20 days. The chimaera was superovulated twice, bred to fertile rams, and the embryos recovered by laparotomy 13 or five days after oestrus, so that karyotype analysis could reveal the genotype of the oocyte. After the first superovulation one ovine day-13 embryo was collected; two fragments of another embryo (or embryos) were also collected, but readable chromosome spreads were not obtained from these embryos or from the two four-cell embryos that were collected five days after the second superovulation. Two surgical embryo transfers to the chimaera resulted in pregnancies. The first transfer involved an eight-cell ovine embryo and two caprine morulae and ended in the abortion of an ovine fetus between days 110 and 130. The second pregnancy occurred after the transfer of two ovine and two caprine morulae. A healthy lamb was born on day 147 of pregnancy. Both placentae had small numbers of cotyledons. A histological evaluation of the cotyledons revealed an abnormal placentome structure in the first pregnancy but not in the second.

Original languageEnglish (US)
Pages (from-to)467-469
Number of pages3
JournalThe Veterinary record
Volume129
Issue number21
DOIs
StatePublished - Jan 1 1991

Fingerprint

chimerism
Goats
Reproduction
Sheep
embryo (animal)
Embryonic Structures
goats
sheep
pregnancy
Superovulation
Morula
Pregnancy
superovulation
Cotyledon
morula
placenta
rams
Placenta
cotyledons
abortion (animals)

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Preliminary observations on reproduction in a female sheep-goat chimaera. / Anderson, G. B.; Ruffing, N. A.; Bondurant, Robert; Pashen, R. L.

In: The Veterinary record, Vol. 129, No. 21, 01.01.1991, p. 467-469.

Research output: Contribution to journalArticle

Anderson, G. B. ; Ruffing, N. A. ; Bondurant, Robert ; Pashen, R. L. / Preliminary observations on reproduction in a female sheep-goat chimaera. In: The Veterinary record. 1991 ; Vol. 129, No. 21. pp. 467-469.
@article{b2203214bb314cd89cfc86d868ee011f,
title = "Preliminary observations on reproduction in a female sheep-goat chimaera.",
abstract = "Four oestrous cycles of a female sheep-goat chimaera were monitored by using a vasectomised ram. The mean (+/- se) length of the cycle was 18.5 +/- 0.64 days with a range from 17 to 20 days. The chimaera was superovulated twice, bred to fertile rams, and the embryos recovered by laparotomy 13 or five days after oestrus, so that karyotype analysis could reveal the genotype of the oocyte. After the first superovulation one ovine day-13 embryo was collected; two fragments of another embryo (or embryos) were also collected, but readable chromosome spreads were not obtained from these embryos or from the two four-cell embryos that were collected five days after the second superovulation. Two surgical embryo transfers to the chimaera resulted in pregnancies. The first transfer involved an eight-cell ovine embryo and two caprine morulae and ended in the abortion of an ovine fetus between days 110 and 130. The second pregnancy occurred after the transfer of two ovine and two caprine morulae. A healthy lamb was born on day 147 of pregnancy. Both placentae had small numbers of cotyledons. A histological evaluation of the cotyledons revealed an abnormal placentome structure in the first pregnancy but not in the second.",
author = "Anderson, {G. B.} and Ruffing, {N. A.} and Robert Bondurant and Pashen, {R. L.}",
year = "1991",
month = "1",
day = "1",
doi = "10.1136/vr.129.21.467",
language = "English (US)",
volume = "129",
pages = "467--469",
journal = "Veterinary Record",
issn = "0042-4900",
publisher = "British Veterinary Association",
number = "21",

}

TY - JOUR

T1 - Preliminary observations on reproduction in a female sheep-goat chimaera.

AU - Anderson, G. B.

AU - Ruffing, N. A.

AU - Bondurant, Robert

AU - Pashen, R. L.

PY - 1991/1/1

Y1 - 1991/1/1

N2 - Four oestrous cycles of a female sheep-goat chimaera were monitored by using a vasectomised ram. The mean (+/- se) length of the cycle was 18.5 +/- 0.64 days with a range from 17 to 20 days. The chimaera was superovulated twice, bred to fertile rams, and the embryos recovered by laparotomy 13 or five days after oestrus, so that karyotype analysis could reveal the genotype of the oocyte. After the first superovulation one ovine day-13 embryo was collected; two fragments of another embryo (or embryos) were also collected, but readable chromosome spreads were not obtained from these embryos or from the two four-cell embryos that were collected five days after the second superovulation. Two surgical embryo transfers to the chimaera resulted in pregnancies. The first transfer involved an eight-cell ovine embryo and two caprine morulae and ended in the abortion of an ovine fetus between days 110 and 130. The second pregnancy occurred after the transfer of two ovine and two caprine morulae. A healthy lamb was born on day 147 of pregnancy. Both placentae had small numbers of cotyledons. A histological evaluation of the cotyledons revealed an abnormal placentome structure in the first pregnancy but not in the second.

AB - Four oestrous cycles of a female sheep-goat chimaera were monitored by using a vasectomised ram. The mean (+/- se) length of the cycle was 18.5 +/- 0.64 days with a range from 17 to 20 days. The chimaera was superovulated twice, bred to fertile rams, and the embryos recovered by laparotomy 13 or five days after oestrus, so that karyotype analysis could reveal the genotype of the oocyte. After the first superovulation one ovine day-13 embryo was collected; two fragments of another embryo (or embryos) were also collected, but readable chromosome spreads were not obtained from these embryos or from the two four-cell embryos that were collected five days after the second superovulation. Two surgical embryo transfers to the chimaera resulted in pregnancies. The first transfer involved an eight-cell ovine embryo and two caprine morulae and ended in the abortion of an ovine fetus between days 110 and 130. The second pregnancy occurred after the transfer of two ovine and two caprine morulae. A healthy lamb was born on day 147 of pregnancy. Both placentae had small numbers of cotyledons. A histological evaluation of the cotyledons revealed an abnormal placentome structure in the first pregnancy but not in the second.

UR - http://www.scopus.com/inward/record.url?scp=0026430596&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026430596&partnerID=8YFLogxK

U2 - 10.1136/vr.129.21.467

DO - 10.1136/vr.129.21.467

M3 - Article

VL - 129

SP - 467

EP - 469

JO - Veterinary Record

JF - Veterinary Record

SN - 0042-4900

IS - 21

ER -