Preliminary evaluation of DNA damage related with the smoking habit measured by the comet assay in whole blood cells

Manuel F. Ellahueñe, Luz Patricia Pérez-Alzola, Mauricio Farfán-Urzua, Patricio González-Hormazabal, Marta Garay, Maria Isabel Olmedo, Jerold A Last

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The alkaline single-cell gel electrophoresis (SCGE) assay, also called the comet assay, is a rapid and simple method for the detection of DNA damage in individual cells. The objective of this study was to establish if the alkaline SCGE assay in whole blood cells gives similar results as the same method in isolated lymphocytes, because whole blood cells are simpler and more economical to use, specifically in human genotoxic biomonitoring. To validate the method, we first used mouse blood cells, because mouse is one of the most commonly used animals in genetic toxicology testing. Groups of seven CF1 male mice were given i.p. injections of relatively low doses of methyl methanesulfonate (25 mg/kg body weight), a direct acting genotoxic agent, or cyclophosphamide (50 mg/kg body weight), which requires metabolic activation. Three, 6, 8, 12, 16, 20, and 65 hours after treatment, 5 μL of blood were collected from each animal and were processed for the alkaline SCGE assay. On the basis of an analysis of tail moment, the results showed that this assay can detect DNA damage induced by both kinds of alkylating mutagens. We then did a preliminary study to assess the status of DNA damage in a young (19 to 23 years old) healthy population of male smokers (n = 6) and nonsmokers (n = 6) using the comet assay in whole blood cells. A significant difference was observed between the two groups, showing that the method is able to detect DNA damage in the smoking group despite the short time that the volunteers had actually been smoking.

Original languageEnglish (US)
Pages (from-to)1223-1229
Number of pages7
JournalCancer Epidemiology Biomarkers and Prevention
Volume13
Issue number7
StatePublished - Jul 2004

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Comet Assay
DNA Damage
Habits
Blood Cells
Smoking
Body Weight
Methyl Methanesulfonate
Environmental Monitoring
Mutagens
Genetic Testing
Cyclophosphamide
Toxicology
Tail
Volunteers
Lymphocytes
Injections
Population

ASJC Scopus subject areas

  • Epidemiology
  • Oncology

Cite this

Ellahueñe, M. F., Pérez-Alzola, L. P., Farfán-Urzua, M., González-Hormazabal, P., Garay, M., Olmedo, M. I., & Last, J. A. (2004). Preliminary evaluation of DNA damage related with the smoking habit measured by the comet assay in whole blood cells. Cancer Epidemiology Biomarkers and Prevention, 13(7), 1223-1229.

Preliminary evaluation of DNA damage related with the smoking habit measured by the comet assay in whole blood cells. / Ellahueñe, Manuel F.; Pérez-Alzola, Luz Patricia; Farfán-Urzua, Mauricio; González-Hormazabal, Patricio; Garay, Marta; Olmedo, Maria Isabel; Last, Jerold A.

In: Cancer Epidemiology Biomarkers and Prevention, Vol. 13, No. 7, 07.2004, p. 1223-1229.

Research output: Contribution to journalArticle

Ellahueñe, MF, Pérez-Alzola, LP, Farfán-Urzua, M, González-Hormazabal, P, Garay, M, Olmedo, MI & Last, JA 2004, 'Preliminary evaluation of DNA damage related with the smoking habit measured by the comet assay in whole blood cells', Cancer Epidemiology Biomarkers and Prevention, vol. 13, no. 7, pp. 1223-1229.
Ellahueñe MF, Pérez-Alzola LP, Farfán-Urzua M, González-Hormazabal P, Garay M, Olmedo MI et al. Preliminary evaluation of DNA damage related with the smoking habit measured by the comet assay in whole blood cells. Cancer Epidemiology Biomarkers and Prevention. 2004 Jul;13(7):1223-1229.
Ellahueñe, Manuel F. ; Pérez-Alzola, Luz Patricia ; Farfán-Urzua, Mauricio ; González-Hormazabal, Patricio ; Garay, Marta ; Olmedo, Maria Isabel ; Last, Jerold A. / Preliminary evaluation of DNA damage related with the smoking habit measured by the comet assay in whole blood cells. In: Cancer Epidemiology Biomarkers and Prevention. 2004 ; Vol. 13, No. 7. pp. 1223-1229.
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