Thirteen baboon embryos were collected at various times before implantation and classified into three stages of development, based on a correlation of morphological development and estimated fertilization age. The fertilization age was estimated by designating the day of mating and/or expected ovulation as day zero. Females were mated with males of proven fertility three days before the estimated onset of perineal deturgescence based on previous cycles. All stages were collected by using an in situ flushing procedure or by flushing the excised oviduct and/or uterus. The two zygotes comprising stage I were recovered from the oviducts on the first day after fertilization. Stage II contains six embryos ranging in development from four to aproximately 32 cells. They were recovered from the oviducts and uteri on the second to the sixth days after fertilization. Stage III consists of five embryos in the free blastocyst stage which were recovered from uterine washings on the fifth to the eighth day after fertilization. One free blastocyst was recovered with the zona pellucida intact, another was in the process of shedding the zona pellucida, and two blastocysts were recovered after the zona pellucida had been shed. The fifth blastocyst was lost before it could be studied in detail.
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics