Pregnancy Detection in Bighorn Sheep (Ovis Canadensis) Using a Fecal-based Enzyme Immunoassay

Dori L Borjesson, Walter M Boyce, Ian Gardner, James DeForge, Bill Lasley

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We developed and validated an enzyme immunoassay for immunoreactive pregnanediol-3-glucuronide (iPdG) in feces to monitor reproductive status in desert and Rocky Mountain bighorn sheep (Ovis canadensis). Fecal iPdG concentrations were strongly correlated (r = 0.71) with serum progesterone concentrations in paired fecal and blood samples collected from 34 free-ranging desert bighorn sheep. In bimonthly fecal samples collected from 12 captive ewes, fecal iPdG profiles were similar between desert and Rocky Mountain bighorn sheep and we selected a pregnancy detection cutoff value of iPdG ≥1.8 ng/mg feces. Fecal iPdG concentrations always exceeded this cutoff value when samples were collected from about day 60 of pregnancy to a few days before parturition, but values <1.8 ng/mg (false negatives) were common for samples collected during the first 60 days of gestation. Although we tested a small number of known pregnant and non-pregnant ewes, the accuracy of the assay was 100% when two samples, collected 2 wk apart, were evaluated for any given ewe. Based on these data, this direct enzyme immunoassay for fecal progesterone metabolites has promise as a diagnostic tool to monitor hormone excretion and pregnancy in a free-ranging ungulate species.

Original languageEnglish (US)
Pages (from-to)67-74
Number of pages8
JournalJournal of Wildlife Diseases
Volume32
Issue number1
StatePublished - Jan 1996

Fingerprint

Bighorn Sheep
pregnanediol
Ovis canadensis
pregnancy diagnosis
immunoassay
enzyme immunoassays
Immunoenzyme Techniques
pregnancy
sheep
enzyme
Pregnancy
desert
deserts
ewes
feces
Rocky Mountain region
Feces
Progesterone
progesterone
sampling

Keywords

  • Bighorn sheep
  • Fecal steroids
  • Immunoassay
  • Pregnancy
  • Progesterone
  • Reproduction

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Pregnancy Detection in Bighorn Sheep (Ovis Canadensis) Using a Fecal-based Enzyme Immunoassay. / Borjesson, Dori L; Boyce, Walter M; Gardner, Ian; DeForge, James; Lasley, Bill.

In: Journal of Wildlife Diseases, Vol. 32, No. 1, 01.1996, p. 67-74.

Research output: Contribution to journalArticle

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