Predictive assessment of kidney functional recovery following ischemic injury using optical spectroscopy

Rajesh N. Raman, Christopher D. Pivetti, Rajendra Ramsamooj, Christoph Troppmann, Stavros G. Demos

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Functional changes in rat kidneys during the induced ischemic injury and recovery phases were explored using multimodal autofluorescence and light scattering imaging. The aim is to evaluate the use of noncontact optical signatures for rapid assessment of tissue function and viability. Specifically, autofluorescence images were acquired in vivo under 355, 325, and 266 nm illumination while light scattering images were collected at the excitation wavelengths as well as using relatively narrowband light centered at 500 nm. The images were simultaneously recorded using a multimodal optical imaging system. The signals were analyzed to obtain time constants, which were correlated to kidney dysfunction as determined by a subsequent survival study and histopathological analysis. Analysis of both the light scattering and autofluorescence images suggests that changes in tissue microstructure, fluorophore emission, and blood absorption spectral characteristics, coupled with vascular response, contribute to the behavior of the observed signal, which may be used to obtain tissue functional information and offer the ability to predict posttransplant kidney function.

Original languageEnglish (US)
Article number056001
JournalJournal of Biomedical Optics
Volume22
Issue number5
DOIs
StatePublished - 2017

Keywords

  • In vivo
  • Ischemia
  • Kidney
  • Reperfusion
  • Spectral imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Biomedical Engineering

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