Precocious retinal neurons: Pax6 controls timing of differentiation and determination of cell type

Gary T. Philips, Carrie N. Stair, Hae Young Lee, Emily Wroblewski, Michael A. Berberoglu, Nadean L Brown, Grant S. Mastick

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

The transcription factor Pax6 plays a pivotal role in eye development, as eye morphogenesis is arrested at a primitive optic vesicle stage in homozygous Pax6 mutant mouse embryos. The arrested optic vesicle development has led to the assumption that cellular differentiation programs are unable to initiate. Contrary to this, we found that neurogenesis in Pax6 mutant optic vesicles was not arrested, but instead accelerated as numerous neurons differentiated precociously, more than a day earlier than normal. To identify potential mechanisms for Pax6 repression of neuron differentiation, we examined retinal proliferation and differentiation. Mutant optic vesicles had reduced proliferation, coupled with precocious activation of the proneural gene, Mash1. Ectopic expression of Mash1 was sufficient to induce precocious neuron differentiation. Subsequently, precocious neurons adopted a generic rather than a specific retinal neuron fate. Thus, Pax6 regulates the timing of retinal neurogenesis and couples it with specific neuron differentiation programs.

Original languageEnglish (US)
Pages (from-to)308-321
Number of pages14
JournalDevelopmental Biology
Volume279
Issue number2
DOIs
StatePublished - Mar 15 2005
Externally publishedYes

Fingerprint

Retinal Neurons
Cell Differentiation
Neurons
Neurogenesis
Morphogenesis
Transcriptional Activation
Transcription Factors
Embryonic Structures

Keywords

  • Aniridia
  • bHLH
  • Brn3b
  • Isl1
  • Mash1
  • Mouse embryo
  • Mutant
  • Neurogenesis
  • Neuron differentiation
  • Proneural
  • Retina
  • Sey
  • Small eye
  • Timing
  • Transcription factor

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Philips, G. T., Stair, C. N., Lee, H. Y., Wroblewski, E., Berberoglu, M. A., Brown, N. L., & Mastick, G. S. (2005). Precocious retinal neurons: Pax6 controls timing of differentiation and determination of cell type. Developmental Biology, 279(2), 308-321. https://doi.org/10.1016/j.ydbio.2004.12.018

Precocious retinal neurons : Pax6 controls timing of differentiation and determination of cell type. / Philips, Gary T.; Stair, Carrie N.; Lee, Hae Young; Wroblewski, Emily; Berberoglu, Michael A.; Brown, Nadean L; Mastick, Grant S.

In: Developmental Biology, Vol. 279, No. 2, 15.03.2005, p. 308-321.

Research output: Contribution to journalArticle

Philips, GT, Stair, CN, Lee, HY, Wroblewski, E, Berberoglu, MA, Brown, NL & Mastick, GS 2005, 'Precocious retinal neurons: Pax6 controls timing of differentiation and determination of cell type', Developmental Biology, vol. 279, no. 2, pp. 308-321. https://doi.org/10.1016/j.ydbio.2004.12.018
Philips, Gary T. ; Stair, Carrie N. ; Lee, Hae Young ; Wroblewski, Emily ; Berberoglu, Michael A. ; Brown, Nadean L ; Mastick, Grant S. / Precocious retinal neurons : Pax6 controls timing of differentiation and determination of cell type. In: Developmental Biology. 2005 ; Vol. 279, No. 2. pp. 308-321.
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