Precise toxigenic ablation of intermediate cells abolishes the "battery" of the cochlear duct

Hyo Jeong Kim, Michael Anne Gratton, Jeong Han Lee, Maria Cristina Perez Flores, Wenying Wang, Karen J. Doyle, Friedrich Beermann, Michael A. Crognale, Ebenezer N. Yamoah

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The extracellular potential of excitable and nonexcitable cells with respect to ground is ~0 mV. One of the known exceptions in mammals is the cochlear duct, where the potential is ~80 -100 mV, called the endocochlear potential (EP). The EP serves as the "battery" for transduction of sound, contributing toward the sensitivity of the auditory system. The stria vascularis (StV) of the cochlear duct is the station where the EP is generated, but the cell-specific roles in the StV are ill defined. Using the intermediate cell (IC)-specific tyrosinase promoter, under the control of diphtheria toxin (DT), we eliminated and/or halted differentiation of neural crest melanocytes after migration to the StV. The ensuing adult transgenic mice are profoundly deaf. Additionally, the EP was abolished. Expression of melanocyte early marker and Kir4.1 in ICs precedes the onset of pigment synthesis. Activation of DT leads to loss of ICs. Finally, in accord with the distinct embryology of retinal pigmented cells, transgenic mice with toxigenic ablation of neural crest-derived melanocytes have intact visual responses. We assert that the tyrosinase promoter is the distinct target for genetic manipulation of IC-specific genes.

Original languageEnglish (US)
Pages (from-to)14601-14606
Number of pages6
JournalJournal of Neuroscience
Volume33
Issue number36
DOIs
StatePublished - 2013

    Fingerprint

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Kim, H. J., Gratton, M. A., Lee, J. H., Perez Flores, M. C., Wang, W., Doyle, K. J., Beermann, F., Crognale, M. A., & Yamoah, E. N. (2013). Precise toxigenic ablation of intermediate cells abolishes the "battery" of the cochlear duct. Journal of Neuroscience, 33(36), 14601-14606. https://doi.org/10.1523/JNEUROSCI.2147-13.2013