Precise monitoring of chemical changes through localization analysis of dynamic spectra (LADS)

Zachary J. Smith, Che Wei Chang, Latevi S. Lawson, Stephen M. Lane, Sebastian Wachsmann-Hogiu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We present a method for monitoring subtle (sub-wavenumber) dynamics within time-varying spectra. Peak fitting is performed for large numbers of spectra in a series, allowing for monitoring time evolutions of peak positions with high precision and confidence. Sub-wavenumber peak shifts due to physical or chemical changes in the sample can be monitored and their temporal evolution characterized. In surface-enhanced Raman scattering experiments, we were able to distinguish between slow photo-damage and fast conformational change dynamics. Fluctuations in peak positions of Raman spectra recorded from a single yeast cell indicated that no significant irreversible photo-damage occurred, but these fluctuations suggest changes in the trapping conditions or biochemical changes associated with the cellular machinery in the cell. The technique is particularly suitable for applications where dynamics of spectra are of interest.

Original languageEnglish (US)
Pages (from-to)187-195
Number of pages9
JournalApplied Spectroscopy
Volume67
Issue number2
DOIs
StatePublished - Feb 2013

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Raman scattering
Monitoring
Raman spectra
damage
Yeast
Machinery
yeast
machinery
Cells
confidence
trapping
shift
cells
Experiments

Keywords

  • Curve fitting
  • Raman scattering
  • SERS
  • Spectroscopy
  • Time resolved

ASJC Scopus subject areas

  • Spectroscopy
  • Instrumentation

Cite this

Precise monitoring of chemical changes through localization analysis of dynamic spectra (LADS). / Smith, Zachary J.; Chang, Che Wei; Lawson, Latevi S.; Lane, Stephen M.; Wachsmann-Hogiu, Sebastian.

In: Applied Spectroscopy, Vol. 67, No. 2, 02.2013, p. 187-195.

Research output: Contribution to journalArticle

Smith, Zachary J. ; Chang, Che Wei ; Lawson, Latevi S. ; Lane, Stephen M. ; Wachsmann-Hogiu, Sebastian. / Precise monitoring of chemical changes through localization analysis of dynamic spectra (LADS). In: Applied Spectroscopy. 2013 ; Vol. 67, No. 2. pp. 187-195.
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