Abstract
OBJECTIVE:: The aim of the study was to determine the impact of increasing doses of 2 prebiotic oligosaccharides and of an "all-human diet" on the intestinal microbiota of premature infants. METHODS:: Twelve premature infants receiving formula feedings were randomly assigned to receive either galacto-oligosaccharide (F+GOS) or a pooled concentrated donor human milk product containing human milk oligosaccharides (F+HMO) in increasing doses during a 5-week period. A second group of 15 premature infants received their motherÊs own milk fortified with either a concentrated donor human milk product (H+H) or a bovine powdered fortifier (H+B). Serial stool specimens from each infant were analyzed by terminal restriction fragment length polymorphism and quantitative polymerase chain reaction for bacterial composition. RESULTS:: All of the infants studied had relatively low levels of bifidobacteria and no measurable Lactobacilli. Infants from the F+GOS and F+HMO groups demonstrated an increase in relative numbers of Clostridia with increasing doses. Compared with the H+B group, the infants in the F+HMO and the H+H groups showed an unexpected trend toward an increase in γ-Proteobacteria over time/dose. Principal coordinate analyses and Shannon diversity scores were not significantly different among the 4 groups. Infants in the H+H group received more antibiotics during the study period than those in the other groups. Two of the infants receiving GOS developed feeding intolerance. CONCLUSIONS:: None of the prebiotic interventions resulted in significant increases in bifidobacteria compared with baseline specimens or the H+B group; however, many of the infants did not receive the highest doses of GOS and HMO, and antibiotic use in the H+H group was high.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 352-360 |
| Number of pages | 9 |
| Journal | Journal of Pediatric Gastroenterology and Nutrition |
| Volume | 58 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 2014 |
Fingerprint
Keywords
- All-human diet
- Galacto-oligosaccharide
- Human milk oligosaccharide
- Late-onset sepsis
- Necrotizing enterocolitis
ASJC Scopus subject areas
- Gastroenterology
- Pediatrics, Perinatology, and Child Health
Cite this
Prebiotic oligosaccharides in premature infants. / Underwood, Mark; Kalanetra, Karen M.; Bokulich, Nicholas A.; Mirmiran, Majid; Barile, Daniela; Tancredi, Daniel J; German, J. Bruce; Lebrilla, Carlito B; Mills, David A.
In: Journal of Pediatric Gastroenterology and Nutrition, Vol. 58, No. 3, 03.2014, p. 352-360.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Prebiotic oligosaccharides in premature infants
AU - Underwood, Mark
AU - Kalanetra, Karen M.
AU - Bokulich, Nicholas A.
AU - Mirmiran, Majid
AU - Barile, Daniela
AU - Tancredi, Daniel J
AU - German, J. Bruce
AU - Lebrilla, Carlito B
AU - Mills, David A.
PY - 2014/3
Y1 - 2014/3
N2 - OBJECTIVE:: The aim of the study was to determine the impact of increasing doses of 2 prebiotic oligosaccharides and of an "all-human diet" on the intestinal microbiota of premature infants. METHODS:: Twelve premature infants receiving formula feedings were randomly assigned to receive either galacto-oligosaccharide (F+GOS) or a pooled concentrated donor human milk product containing human milk oligosaccharides (F+HMO) in increasing doses during a 5-week period. A second group of 15 premature infants received their motherÊs own milk fortified with either a concentrated donor human milk product (H+H) or a bovine powdered fortifier (H+B). Serial stool specimens from each infant were analyzed by terminal restriction fragment length polymorphism and quantitative polymerase chain reaction for bacterial composition. RESULTS:: All of the infants studied had relatively low levels of bifidobacteria and no measurable Lactobacilli. Infants from the F+GOS and F+HMO groups demonstrated an increase in relative numbers of Clostridia with increasing doses. Compared with the H+B group, the infants in the F+HMO and the H+H groups showed an unexpected trend toward an increase in γ-Proteobacteria over time/dose. Principal coordinate analyses and Shannon diversity scores were not significantly different among the 4 groups. Infants in the H+H group received more antibiotics during the study period than those in the other groups. Two of the infants receiving GOS developed feeding intolerance. CONCLUSIONS:: None of the prebiotic interventions resulted in significant increases in bifidobacteria compared with baseline specimens or the H+B group; however, many of the infants did not receive the highest doses of GOS and HMO, and antibiotic use in the H+H group was high.
AB - OBJECTIVE:: The aim of the study was to determine the impact of increasing doses of 2 prebiotic oligosaccharides and of an "all-human diet" on the intestinal microbiota of premature infants. METHODS:: Twelve premature infants receiving formula feedings were randomly assigned to receive either galacto-oligosaccharide (F+GOS) or a pooled concentrated donor human milk product containing human milk oligosaccharides (F+HMO) in increasing doses during a 5-week period. A second group of 15 premature infants received their motherÊs own milk fortified with either a concentrated donor human milk product (H+H) or a bovine powdered fortifier (H+B). Serial stool specimens from each infant were analyzed by terminal restriction fragment length polymorphism and quantitative polymerase chain reaction for bacterial composition. RESULTS:: All of the infants studied had relatively low levels of bifidobacteria and no measurable Lactobacilli. Infants from the F+GOS and F+HMO groups demonstrated an increase in relative numbers of Clostridia with increasing doses. Compared with the H+B group, the infants in the F+HMO and the H+H groups showed an unexpected trend toward an increase in γ-Proteobacteria over time/dose. Principal coordinate analyses and Shannon diversity scores were not significantly different among the 4 groups. Infants in the H+H group received more antibiotics during the study period than those in the other groups. Two of the infants receiving GOS developed feeding intolerance. CONCLUSIONS:: None of the prebiotic interventions resulted in significant increases in bifidobacteria compared with baseline specimens or the H+B group; however, many of the infants did not receive the highest doses of GOS and HMO, and antibiotic use in the H+H group was high.
KW - All-human diet
KW - Galacto-oligosaccharide
KW - Human milk oligosaccharide
KW - Late-onset sepsis
KW - Necrotizing enterocolitis
UR - http://www.scopus.com/inward/record.url?scp=84896702139&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84896702139&partnerID=8YFLogxK
U2 - 10.1097/MPG.0000000000000211
DO - 10.1097/MPG.0000000000000211
M3 - Article
C2 - 24135979
AN - SCOPUS:84896702139
VL - 58
SP - 352
EP - 360
JO - Journal of Pediatric Gastroenterology and Nutrition
JF - Journal of Pediatric Gastroenterology and Nutrition
SN - 0277-2116
IS - 3
ER -