Power-line frequency electromagnetic fields do not induce changes in phosphorylation, localization, or expression of the 27-kilodalton heat shock protein in human keratinocytes

Biao Shi, Behnom Farboud, Richard Nuccitelli, Roslyn Rivkah Isseroff

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The linkage of the exposure to the power-line frequency (50-60 Hz) electromagnetic fields (EMF) with human cancers remains controversial after more than 10 years of study. The in vitro studies on the adverse effects of EMF on human cells have not yielded a clear conclusion. In this study, we investigated whether power-line frequency EMF could act as an environmental insult to invoke stress responses in human keratinocytes using the 27-kDa heat shock protein (HSP27) as a stress marker. After exposure to 1 gauss (100 μT) EMF from 20 min to 24 hr, the isoform pattern of HSP27 in keratinocytes remained unchanged, suggesting that EMF did not induce the phosphorylation of this stress protein. EMF exposure also failed to induce the translocation of HSP27 from the cytoplasm to the nucleus. Moreover, EMF exposure did not increase the abundance of HSP27 in keratinocytes. In addition, we found no evidence that EMF exposure enhanced the level of the 70-kDa heat shock protein (HSP70) in breast or leukemia cells as reported previously. Therefore, in this study we did not detect any of a number of stress responses in human keratinocytes exposed to power-line frequency EMF.

Original languageEnglish (US)
Pages (from-to)281-287
Number of pages7
JournalEnvironmental Health Perspectives
Volume111
Issue number3
StatePublished - Mar 1 2003

Fingerprint

HSP27 Heat-Shock Proteins
power line
Phosphorylation
Electromagnetic Fields
heat shock
electromagnetic field
Heat-Shock Proteins
Keratinocytes
Electromagnetic fields
protein
HSP70 Heat-Shock Proteins
cytoplasm
translocation
cancer
Protein Isoforms
Cytoplasm
Leukemia
Breast
Cells
exposure

Keywords

  • Electromagnetic fields
  • Heat shock proteins
  • HSP27 phosphorylation
  • HSP27 translocation
  • HSP70
  • Keratinocytes
  • Signal transduction
  • Stress

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health

Cite this

@article{da701cb91be84023a1a0edc7302b67ce,
title = "Power-line frequency electromagnetic fields do not induce changes in phosphorylation, localization, or expression of the 27-kilodalton heat shock protein in human keratinocytes",
abstract = "The linkage of the exposure to the power-line frequency (50-60 Hz) electromagnetic fields (EMF) with human cancers remains controversial after more than 10 years of study. The in vitro studies on the adverse effects of EMF on human cells have not yielded a clear conclusion. In this study, we investigated whether power-line frequency EMF could act as an environmental insult to invoke stress responses in human keratinocytes using the 27-kDa heat shock protein (HSP27) as a stress marker. After exposure to 1 gauss (100 μT) EMF from 20 min to 24 hr, the isoform pattern of HSP27 in keratinocytes remained unchanged, suggesting that EMF did not induce the phosphorylation of this stress protein. EMF exposure also failed to induce the translocation of HSP27 from the cytoplasm to the nucleus. Moreover, EMF exposure did not increase the abundance of HSP27 in keratinocytes. In addition, we found no evidence that EMF exposure enhanced the level of the 70-kDa heat shock protein (HSP70) in breast or leukemia cells as reported previously. Therefore, in this study we did not detect any of a number of stress responses in human keratinocytes exposed to power-line frequency EMF.",
keywords = "Electromagnetic fields, Heat shock proteins, HSP27 phosphorylation, HSP27 translocation, HSP70, Keratinocytes, Signal transduction, Stress",
author = "Biao Shi and Behnom Farboud and Richard Nuccitelli and Isseroff, {Roslyn Rivkah}",
year = "2003",
month = "3",
day = "1",
language = "English (US)",
volume = "111",
pages = "281--287",
journal = "Environmental Health Perspectives",
issn = "0091-6765",
publisher = "Public Health Services, US Dept of Health and Human Services",
number = "3",

}

TY - JOUR

T1 - Power-line frequency electromagnetic fields do not induce changes in phosphorylation, localization, or expression of the 27-kilodalton heat shock protein in human keratinocytes

AU - Shi, Biao

AU - Farboud, Behnom

AU - Nuccitelli, Richard

AU - Isseroff, Roslyn Rivkah

PY - 2003/3/1

Y1 - 2003/3/1

N2 - The linkage of the exposure to the power-line frequency (50-60 Hz) electromagnetic fields (EMF) with human cancers remains controversial after more than 10 years of study. The in vitro studies on the adverse effects of EMF on human cells have not yielded a clear conclusion. In this study, we investigated whether power-line frequency EMF could act as an environmental insult to invoke stress responses in human keratinocytes using the 27-kDa heat shock protein (HSP27) as a stress marker. After exposure to 1 gauss (100 μT) EMF from 20 min to 24 hr, the isoform pattern of HSP27 in keratinocytes remained unchanged, suggesting that EMF did not induce the phosphorylation of this stress protein. EMF exposure also failed to induce the translocation of HSP27 from the cytoplasm to the nucleus. Moreover, EMF exposure did not increase the abundance of HSP27 in keratinocytes. In addition, we found no evidence that EMF exposure enhanced the level of the 70-kDa heat shock protein (HSP70) in breast or leukemia cells as reported previously. Therefore, in this study we did not detect any of a number of stress responses in human keratinocytes exposed to power-line frequency EMF.

AB - The linkage of the exposure to the power-line frequency (50-60 Hz) electromagnetic fields (EMF) with human cancers remains controversial after more than 10 years of study. The in vitro studies on the adverse effects of EMF on human cells have not yielded a clear conclusion. In this study, we investigated whether power-line frequency EMF could act as an environmental insult to invoke stress responses in human keratinocytes using the 27-kDa heat shock protein (HSP27) as a stress marker. After exposure to 1 gauss (100 μT) EMF from 20 min to 24 hr, the isoform pattern of HSP27 in keratinocytes remained unchanged, suggesting that EMF did not induce the phosphorylation of this stress protein. EMF exposure also failed to induce the translocation of HSP27 from the cytoplasm to the nucleus. Moreover, EMF exposure did not increase the abundance of HSP27 in keratinocytes. In addition, we found no evidence that EMF exposure enhanced the level of the 70-kDa heat shock protein (HSP70) in breast or leukemia cells as reported previously. Therefore, in this study we did not detect any of a number of stress responses in human keratinocytes exposed to power-line frequency EMF.

KW - Electromagnetic fields

KW - Heat shock proteins

KW - HSP27 phosphorylation

KW - HSP27 translocation

KW - HSP70

KW - Keratinocytes

KW - Signal transduction

KW - Stress

UR - http://www.scopus.com/inward/record.url?scp=0344211432&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0344211432&partnerID=8YFLogxK

M3 - Article

C2 - 12611655

AN - SCOPUS:0344211432

VL - 111

SP - 281

EP - 287

JO - Environmental Health Perspectives

JF - Environmental Health Perspectives

SN - 0091-6765

IS - 3

ER -