Power Line Frequency Electromagnetic Fields Do Not Increase the Rate of Protein Synthesis in Human Skin Fibroblasts as Previously Reported

Blao Shi, Roslyn Rivkah Isseroff, Richard Nuccitelli

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Rodemann et al. [Rodemann et al. (1987): Biochem Biophys Res Commun 145:1-9] reported that human skin fibroblasts increase their rate of protein synthesis by as much as over ninefold in response to long term exposure to 20 Hz, 8.4 mT (84 G) magnetic fields. Here we report studies of protein synthesis using an identical cell type, exposure conditions, and the same means of measuring protein synthetic rates. Our initial goal was to determine if the earlier results could be replicated, but we found an inconsistency in the earlier protocol. It exposed cells to [3H]leucine for 6 h prior to measuring incorporation into protein. We found, however, that 24 h is required for [3H]leucine to reach a steady state distribution across the cells' plasma membranes. In addition, we typically measured 100-200 cpm/ thousand cells. This is four- to eightfold higher than the 19-28 cpm/1000 cells previously reported. Using these conditions, we could find no significant difference in protein synthesis rates between control cells and cells exposed for up to three weeks in an identical electromagnetic field. In addition, we investigated the effects of a 60 Hz field since that is the frequency used for electric power distribution in the United States. Again, we could find no significant effect of this field on rates of protein synthesis, even after 21 days of exposure.

Original languageEnglish (US)
Pages (from-to)465-472
Number of pages8
JournalBioelectromagnetics
Volume24
Issue number7
DOIs
StatePublished - Oct 2003

Fingerprint

Electromagnetic Fields
skin (animal)
fibroblasts
Fibroblasts
protein synthesis
Skin
Proteins
cells
Leucine
leucine
Cell Membrane
synthetic proteins
electric power
Magnetic Fields
chronic exposure
magnetic fields
electromagnetic field
plasma membrane

Keywords

  • EMF
  • Fibroblasts, 3H-leucine incorporation, transmembrane diffusion time
  • Protein synthesis rates
  • Skin

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics

Cite this

Power Line Frequency Electromagnetic Fields Do Not Increase the Rate of Protein Synthesis in Human Skin Fibroblasts as Previously Reported. / Shi, Blao; Isseroff, Roslyn Rivkah; Nuccitelli, Richard.

In: Bioelectromagnetics, Vol. 24, No. 7, 10.2003, p. 465-472.

Research output: Contribution to journalArticle

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