TY - JOUR
T1 - Potentiation of adenosine A1 receptor agonist CPA-induced antinociception by paeoniflorin in mice
AU - Liu, Da
AU - Zhao, Fei Li
AU - Liu, Jing
AU - Li, Xin Quan
AU - Ye, Yang
AU - Zhu, Xing Zu
PY - 2006/8/9
Y1 - 2006/8/9
N2 - The effect of paeoniflorin (PF), a major constituent isolated from Paeony radix, on N6-Cyclopentyladenosine (CPA), a selective adenosine A 1 receptor (A1 receptor) agonist, induced antinociception was examined in mice. In the tail-pressure test, CPA (0.05, 0.1, 0.2 mg/kg, s.c.) could induce antinociception in a dose-dependent manner. PF (5, 10, 20 mg/kg, s.c.) alone failed to exhibit any antinociceptive effect in mice; however, pretreatment of PF (20 mg/kg, s.c.) could significantly enhance CPA-induced antinociception. Additionally, pretreatment of 8-Cyclopentyl-1,3- dipropylxanthine (DPCPX, 0.25 mg/kg, s.c.), a selective A1 receptor antagonist, could antagonize the antinociceptive effect of combining CPA with PF. Furthermore, in the competitive binding experiments, PF did not displace the binding of [3H]-8-Cyclopentyl-1,3-dipropylxanthine ([ 3H]-DPCPX) but displaced that of [3H]-2-Chloro-N 6-cyclopentyladenosine ([3H]-CCPA, a selective A 1 receptor agonist) to the membrane preparation of rat cerebral cortex. These results suggested that PF might selectively increase the binding and antinociceptive effect of CPA by binding with A1 receptor.
AB - The effect of paeoniflorin (PF), a major constituent isolated from Paeony radix, on N6-Cyclopentyladenosine (CPA), a selective adenosine A 1 receptor (A1 receptor) agonist, induced antinociception was examined in mice. In the tail-pressure test, CPA (0.05, 0.1, 0.2 mg/kg, s.c.) could induce antinociception in a dose-dependent manner. PF (5, 10, 20 mg/kg, s.c.) alone failed to exhibit any antinociceptive effect in mice; however, pretreatment of PF (20 mg/kg, s.c.) could significantly enhance CPA-induced antinociception. Additionally, pretreatment of 8-Cyclopentyl-1,3- dipropylxanthine (DPCPX, 0.25 mg/kg, s.c.), a selective A1 receptor antagonist, could antagonize the antinociceptive effect of combining CPA with PF. Furthermore, in the competitive binding experiments, PF did not displace the binding of [3H]-8-Cyclopentyl-1,3-dipropylxanthine ([ 3H]-DPCPX) but displaced that of [3H]-2-Chloro-N 6-cyclopentyladenosine ([3H]-CCPA, a selective A 1 receptor agonist) to the membrane preparation of rat cerebral cortex. These results suggested that PF might selectively increase the binding and antinociceptive effect of CPA by binding with A1 receptor.
KW - Adenosine
KW - Adenosine A receptor
KW - Antinociception
KW - Paeoniflorin
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U2 - 10.1248/bpb.29.1630
DO - 10.1248/bpb.29.1630
M3 - Article
C2 - 16880617
AN - SCOPUS:33746765075
VL - 29
SP - 1630
EP - 1633
JO - Biological and Pharmaceutical Bulletin
JF - Biological and Pharmaceutical Bulletin
SN - 0918-6158
IS - 8
ER -