Potentiation of adenosine A1 receptor agonist CPA-induced antinociception by paeoniflorin in mice

Da Liu, Fei Li Zhao, Jing Liu, Xin Quan Li, Yang Ye, Xing Zu Zhu

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The effect of paeoniflorin (PF), a major constituent isolated from Paeony radix, on N6-Cyclopentyladenosine (CPA), a selective adenosine A 1 receptor (A1 receptor) agonist, induced antinociception was examined in mice. In the tail-pressure test, CPA (0.05, 0.1, 0.2 mg/kg, s.c.) could induce antinociception in a dose-dependent manner. PF (5, 10, 20 mg/kg, s.c.) alone failed to exhibit any antinociceptive effect in mice; however, pretreatment of PF (20 mg/kg, s.c.) could significantly enhance CPA-induced antinociception. Additionally, pretreatment of 8-Cyclopentyl-1,3- dipropylxanthine (DPCPX, 0.25 mg/kg, s.c.), a selective A1 receptor antagonist, could antagonize the antinociceptive effect of combining CPA with PF. Furthermore, in the competitive binding experiments, PF did not displace the binding of [3H]-8-Cyclopentyl-1,3-dipropylxanthine ([ 3H]-DPCPX) but displaced that of [3H]-2-Chloro-N 6-cyclopentyladenosine ([3H]-CCPA, a selective A 1 receptor agonist) to the membrane preparation of rat cerebral cortex. These results suggested that PF might selectively increase the binding and antinociceptive effect of CPA by binding with A1 receptor.

Original languageEnglish (US)
Pages (from-to)1630-1633
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume29
Issue number8
DOIs
StatePublished - Aug 9 2006
Externally publishedYes

Keywords

  • Adenosine
  • Adenosine A receptor
  • Antinociception
  • Paeoniflorin

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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