Posttraumatic lymphocyte response: A comparison between peripheral blood T cells and tissue T cells

Michael M. Aguilar, Felix D. Battistella, John T Owings, Steve A. Olson, Kathleen MacColl

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: T-cell response to trauma has been assessed primarily by sampling peripheral blood lymphocytes. We hypothesized that lymphocytes residing in tissue and traveling through lymph vessels are more likely to be activated by tissue injury and hemorrhage-induced hypoperfusion. We compared peripheral blood T-cell response with tissue or lymph T-cell response in an ovine model of multiple injury. Methods: Anesthetized adult sheep instrumented with a chronic prefemoral lymph fistula were subjected to lower- extremity fractures, fixed-volume hemorrhage, resuscitation, and fracture stabilization. Peripheral blood and tissue T-cell receptor expression was determined at baseline and after injury. Results: At baseline, we found significant differences in the expression of CD4, CD8, and L selectin between peripheral blood T cells and tissue T cells. After trauma, the percentage of tissue T cells expressing CD8 decreased from 19 ± 9 to 14 ± 5 (p < 0.05) and the percentage expressing γδ-TcR receptors decreased from 12 ± 4 to 7 ± 2 (p < 0.05). T-cell phenotype composition in peripheral blood was not affected by trauma. Conclusion: Peripheral blood T-cell composition differs from tissue T-cell composition before and after trauma. Trauma produced changes in tissue T-cell phenotypes but not in peripheral blood T-cell phenotypes.

Original languageEnglish (US)
Pages (from-to)14-18
Number of pages5
JournalJournal of Trauma - Injury, Infection and Critical Care
Volume45
Issue number1
DOIs
StatePublished - 1998

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Blood Cells
Lymphocytes
T-Lymphocytes
Wounds and Injuries
Lymph
Phenotype
Sheep
Hemorrhage
L-Selectin
Multiple Trauma
T-Cell Antigen Receptor
Resuscitation
Fistula
Lower Extremity

Keywords

  • CD4
  • CD8
  • Flow cytometry
  • L selectin
  • Lymphatic lymphocyte
  • Major histocompatibility complex II
  • Peripheral blood lymphocyte
  • Prefemoral lymph fistula
  • T cell
  • Tissue lymphocyte
  • Trauma
  • γδ-TcR

ASJC Scopus subject areas

  • Surgery

Cite this

Posttraumatic lymphocyte response : A comparison between peripheral blood T cells and tissue T cells. / Aguilar, Michael M.; Battistella, Felix D.; Owings, John T; Olson, Steve A.; MacColl, Kathleen.

In: Journal of Trauma - Injury, Infection and Critical Care, Vol. 45, No. 1, 1998, p. 14-18.

Research output: Contribution to journalArticle

Aguilar, Michael M. ; Battistella, Felix D. ; Owings, John T ; Olson, Steve A. ; MacColl, Kathleen. / Posttraumatic lymphocyte response : A comparison between peripheral blood T cells and tissue T cells. In: Journal of Trauma - Injury, Infection and Critical Care. 1998 ; Vol. 45, No. 1. pp. 14-18.
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