Postprandial lipoprotein metabolism

VLDL vs chylomicrons

Katsuyuki Nakajima, Takamitsu Nakano, Yoshiharu Tokita, Takeaki Nagamine, Akihiro Inazu, Junji Kobayashi, Hiroshi Mabuchi, Kimber Stanhope, Peter J Havel, Mitsuyo Okazaki, Masumi Ai, Akira Tanaka

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Since Zilversmit first proposed postprandial lipemia as the most common risk of cardiovascular disease, chylomicrons (CM) and CM remnants have been thought to be the major lipoproteins which are increased in the postprandial hyperlipidemia. However, it has been shown over the last two decades that the major increase in the postprandial lipoproteins after food intake occurs in the very low density lipoprotein (VLDL) remnants (apoB-100 particles), not CM or CM remnants (apoB-48 particles). This finding was obtained using the following three analytical methods; isolation of remnant-like lipoprotein particles (RLP) with specific antibodies, separation and detection of lipoprotein subclasses by gel permeation HPLC and determination of apoB-48 in fractionated lipoproteins by a specific ELISA. The amount of the apoB-48 particles in the postprandial RLP is significantly less than the apoB-100 particles, and the particle sizes of apoB-48 and apoB-100 in RLP are very similar when analyzed by HPLC. Moreover, CM or CM remnants having a large amount of TG were not found in the postprandial RLP. Therefore, the major portion of the TG which is increased in the postprandial state is composed of VLDL remnants, which have been recognized as a significant risk for cardiovascular disease.

Original languageEnglish (US)
Pages (from-to)1306-1318
Number of pages13
JournalClinica Chimica Acta
Volume412
Issue number15-16
DOIs
StatePublished - Jul 15 2011

Fingerprint

Chylomicrons
VLDL Lipoproteins
Metabolism
Lipoproteins
Apolipoprotein B-48
Chylomicron Remnants
Apolipoprotein B-100
Hyperlipidemias
Cardiovascular Diseases
High Pressure Liquid Chromatography
Particle Size
Permeation
Eating
Gels
Enzyme-Linked Immunosorbent Assay
Particle size
Antibodies

Keywords

  • ApoB-100
  • ApoB-48
  • Chylomicron remnants
  • Postprandial remnant lipoproteins
  • Remnant-like lipoprotein particles (RLP)
  • Very low density lipoprotein (VLDL) remnants

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Nakajima, K., Nakano, T., Tokita, Y., Nagamine, T., Inazu, A., Kobayashi, J., ... Tanaka, A. (2011). Postprandial lipoprotein metabolism: VLDL vs chylomicrons. Clinica Chimica Acta, 412(15-16), 1306-1318. https://doi.org/10.1016/j.cca.2011.04.018

Postprandial lipoprotein metabolism : VLDL vs chylomicrons. / Nakajima, Katsuyuki; Nakano, Takamitsu; Tokita, Yoshiharu; Nagamine, Takeaki; Inazu, Akihiro; Kobayashi, Junji; Mabuchi, Hiroshi; Stanhope, Kimber; Havel, Peter J; Okazaki, Mitsuyo; Ai, Masumi; Tanaka, Akira.

In: Clinica Chimica Acta, Vol. 412, No. 15-16, 15.07.2011, p. 1306-1318.

Research output: Contribution to journalArticle

Nakajima, K, Nakano, T, Tokita, Y, Nagamine, T, Inazu, A, Kobayashi, J, Mabuchi, H, Stanhope, K, Havel, PJ, Okazaki, M, Ai, M & Tanaka, A 2011, 'Postprandial lipoprotein metabolism: VLDL vs chylomicrons', Clinica Chimica Acta, vol. 412, no. 15-16, pp. 1306-1318. https://doi.org/10.1016/j.cca.2011.04.018
Nakajima K, Nakano T, Tokita Y, Nagamine T, Inazu A, Kobayashi J et al. Postprandial lipoprotein metabolism: VLDL vs chylomicrons. Clinica Chimica Acta. 2011 Jul 15;412(15-16):1306-1318. https://doi.org/10.1016/j.cca.2011.04.018
Nakajima, Katsuyuki ; Nakano, Takamitsu ; Tokita, Yoshiharu ; Nagamine, Takeaki ; Inazu, Akihiro ; Kobayashi, Junji ; Mabuchi, Hiroshi ; Stanhope, Kimber ; Havel, Peter J ; Okazaki, Mitsuyo ; Ai, Masumi ; Tanaka, Akira. / Postprandial lipoprotein metabolism : VLDL vs chylomicrons. In: Clinica Chimica Acta. 2011 ; Vol. 412, No. 15-16. pp. 1306-1318.
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