Postprandial lipemia is associated with platelet and monocyte activation and increased monocyte cytokine expression in normolipemic men

Dianne A. Hyson, Teresa G. Paglieroni, Theodore Wun, John C Rutledge

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

The activation of platelets and monocytes has been implicated in the development of cardiovascular diseases. We asked the question if postprandial lipemia following a fat-containing meal is associated with platelet and monocyte activation and increased platelet-monocyte interaction. Thirteen healthy, normal weight, normolipemic males, 20 to 49 years, consumed a 40% fat meal of whole foods. Blood samples were obtained at fasting and 3 1/2 and 6 hours after ingestion. Triglyceride levels increased to 48% over baseline at 3 1/2 hours postconsumption and returned to fasting levels by 6 hours. Multiparameter flow cytometry using monoclonal antibodies showed that the percentage of platelets expressing surface P-selectin and the activated conformation the GPIIb-IIIa receptor was significantly higher at 3 1/2 hours compared to fasting. The percentage of platelet-monocyte aggregates increased by 36% at 3 1/2 hours and 43% at 6 hours postconsumption. The percentage of monocytes expressing intracellular tumor necrosis factor-α (TNF-α) increased seven and eightfold at 3 1/2 and 6 hours, respectively. The expression of interleukin-1β (IL-1β increased in a similar manner. These data suggest activation of platelets and monocytes after a moderate fat meal. Repetitive activation of platelets and monocytes could be an early event in the initiation and development of atherosclerosis.

Original languageEnglish (US)
Pages (from-to)147-155
Number of pages9
JournalClinical and Applied Thrombosis/Hemostasis
Volume8
Issue number2
StatePublished - 2002

Fingerprint

Platelet Activation
Hyperlipidemias
Monocytes
Cytokines
Meals
Fasting
Blood Platelets
Fats
Interleukin-1
Platelet Glycoprotein GPIIb-IIIa Complex
P-Selectin
Atherosclerosis
Flow Cytometry
Triglycerides
Cardiovascular Diseases
Tumor Necrosis Factor-alpha
Eating
Monoclonal Antibodies
Weights and Measures
Food

Keywords

  • Cytokines
  • Monocytes
  • Platelets
  • Postprandial
  • Triglyceride

ASJC Scopus subject areas

  • Hematology

Cite this

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abstract = "The activation of platelets and monocytes has been implicated in the development of cardiovascular diseases. We asked the question if postprandial lipemia following a fat-containing meal is associated with platelet and monocyte activation and increased platelet-monocyte interaction. Thirteen healthy, normal weight, normolipemic males, 20 to 49 years, consumed a 40{\%} fat meal of whole foods. Blood samples were obtained at fasting and 3 1/2 and 6 hours after ingestion. Triglyceride levels increased to 48{\%} over baseline at 3 1/2 hours postconsumption and returned to fasting levels by 6 hours. Multiparameter flow cytometry using monoclonal antibodies showed that the percentage of platelets expressing surface P-selectin and the activated conformation the GPIIb-IIIa receptor was significantly higher at 3 1/2 hours compared to fasting. The percentage of platelet-monocyte aggregates increased by 36{\%} at 3 1/2 hours and 43{\%} at 6 hours postconsumption. The percentage of monocytes expressing intracellular tumor necrosis factor-α (TNF-α) increased seven and eightfold at 3 1/2 and 6 hours, respectively. The expression of interleukin-1β (IL-1β increased in a similar manner. These data suggest activation of platelets and monocytes after a moderate fat meal. Repetitive activation of platelets and monocytes could be an early event in the initiation and development of atherosclerosis.",
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