TY - JOUR
T1 - Postnatal development of T cells in dogs with X-linked severe combined immunodeficiency
AU - Somberg, Richard L.
AU - Tipold, Andrea
AU - Hartnett, Brian J.
AU - Moore, Peter F
AU - Henthorn, Paula S.
AU - Felsburg, Peter J.
PY - 1996/2/15
Y1 - 1996/2/15
N2 - X-linked severe combined immunodeficiency disease (XSCID) in both humans and dogs results from mutations in the common γ-chain, γc, which is a common component of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15. Although human and canine XSCID share similar features, such as a failure to thrive, hypogammaglobulinemia, an absent T cell mitogenic response, and thymic dysplasia, near normal percentages of T cells are observed in some affected dogs, whereas XSCID boys have few, if any, circulating T cells. In this study, PBL were analyzed by flow cytometry beginning shortly after birth until 9 wk of age. XSCID dogs <3 wk of age had an elevated number of B cells and were nearly devoid of T cells, phenotypically resembling most human XSCID patients. At 5 wk of age, however, T cells appeared in approximately one- half of the XSCID dogs, although the absolute number of T cells was one- third of normal in these dogs. While the percentage of CD45RA+ T cells in normal dogs gradually decreased with age from >90% in neonates to <40% by 3 to 5 yr of age, in XSCID dogs a rapid decline in the percentage of CD45RA+ T cells was observed, resulting in <10% CD45RA+ T cells by 7 to 9 wk of age. Maternal engraftment was not detected in any of the XSCID dogs by using a sensitive PCR assay. The appearance of nonmaternally derived T cells in XSCID dogs that undergo a rapid switch from CD45RA+ to CD45RA- suggests that limited thymic emigration and peripheral expansion of T cells can occur in the absence of a functional γc.
AB - X-linked severe combined immunodeficiency disease (XSCID) in both humans and dogs results from mutations in the common γ-chain, γc, which is a common component of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15. Although human and canine XSCID share similar features, such as a failure to thrive, hypogammaglobulinemia, an absent T cell mitogenic response, and thymic dysplasia, near normal percentages of T cells are observed in some affected dogs, whereas XSCID boys have few, if any, circulating T cells. In this study, PBL were analyzed by flow cytometry beginning shortly after birth until 9 wk of age. XSCID dogs <3 wk of age had an elevated number of B cells and were nearly devoid of T cells, phenotypically resembling most human XSCID patients. At 5 wk of age, however, T cells appeared in approximately one- half of the XSCID dogs, although the absolute number of T cells was one- third of normal in these dogs. While the percentage of CD45RA+ T cells in normal dogs gradually decreased with age from >90% in neonates to <40% by 3 to 5 yr of age, in XSCID dogs a rapid decline in the percentage of CD45RA+ T cells was observed, resulting in <10% CD45RA+ T cells by 7 to 9 wk of age. Maternal engraftment was not detected in any of the XSCID dogs by using a sensitive PCR assay. The appearance of nonmaternally derived T cells in XSCID dogs that undergo a rapid switch from CD45RA+ to CD45RA- suggests that limited thymic emigration and peripheral expansion of T cells can occur in the absence of a functional γc.
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M3 - Article
C2 - 8568244
AN - SCOPUS:0030023050
VL - 156
SP - 1431
EP - 1435
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 4
ER -