Post prandial plasma free arginine concentrations increase in rainbow trout fed arginine-deficient diets

Gunjun Park, Sungchul C. Bai, Im Ho Ok, Kyungmin Han, Silas S.O. Hung, Quinton Rogers, Taesun Min

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Three experiments were conducted to determine the effects of dietary arginine concentrations on plasma free amino acid (PAA) concentrations in rainbow trout, Oncorhynchus mykiss (Walbaum). The first experiment was conducted to determine appropriate post-prandial and food deprivation sampling times in dorsal aorta cannulated rainbow trout averaging 519±9.5 g (mean±SD) at 16°C. Blood samples were taken at 0, 2, 3, 4, 5, 6 and 24 h after feeding (0 and 24 h blood samples were taken from the same group of fish). PAA concentrations increased by 2 h post-feeding and the concentration of all essential amino acids except histidine peaked at 5 h and returned to 0 time values by 24 h. In the second experiment dorsal aorta cannulated rainbow trout averaging 528±11.3 g (mean±SD) were divided into 6 groups of 4 fish to study the effect of dietary arginine levels on PAA. After 24 h food deprivation, each group of fish was fed one of six L-amino acid diets containing graded levels of arginine (0.48, 1.08, 1.38, 1.68, 1.98 or 2.58%) by intubation. Blood samples were taken at 0, 5 and 24 h after feeding. Post-prandial (5 h after feeding) plasma-free arginine concentrations (PParg) showed a breakpoint at 1.03% arginine in the diet and post-absorptive (24 h after feeding) plasma free-arginine concentrations (PAarg) showed a breakpoint at 1.38% arginine. PAarg increased linearly from fish fed diets containing arginine between 0.48% and 1.38%, and the concentrations remained constant from fish fed diets containing arginine at or above 1.38%, but were all below PParg at all time points. Results of the third experiment confirm the results that PParg concentrations from fish fed arginine deficient diets were higher than PAarg (0 or 24 h values). Thus, in contrast to mammals and birds, the PParg when arginine is present in the diet as the most limiting amino acid such that it severely limits growth, increases in plasma rather than decreases.

Original languageEnglish (US)
Pages (from-to)396-402
Number of pages7
JournalAsian-Australasian Journal of Animal Sciences
Volume18
Issue number3
DOIs
StatePublished - Jan 1 2005

Fingerprint

Oncorhynchus mykiss
arginine
Meals
Arginine
Diet
diet
Fishes
Amino Acids
free amino acids
fish feeds
Food Deprivation
food deprivation
aorta
Aorta
blood
fish
sampling
Essential Amino Acids
limiting amino acids
essential amino acids

Keywords

  • Arginine
  • Dorsal aorta cannulation
  • Plasma free amino acids
  • Rainbow trout

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology

Cite this

Post prandial plasma free arginine concentrations increase in rainbow trout fed arginine-deficient diets. / Park, Gunjun; Bai, Sungchul C.; Ok, Im Ho; Han, Kyungmin; Hung, Silas S.O.; Rogers, Quinton; Min, Taesun.

In: Asian-Australasian Journal of Animal Sciences, Vol. 18, No. 3, 01.01.2005, p. 396-402.

Research output: Contribution to journalArticle

Park, Gunjun ; Bai, Sungchul C. ; Ok, Im Ho ; Han, Kyungmin ; Hung, Silas S.O. ; Rogers, Quinton ; Min, Taesun. / Post prandial plasma free arginine concentrations increase in rainbow trout fed arginine-deficient diets. In: Asian-Australasian Journal of Animal Sciences. 2005 ; Vol. 18, No. 3. pp. 396-402.
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