A DNA cleavage reagent, specifically tethered to residue 581 of the Escherichia coli RNA polymerase σ70 subunit, has been used to investigate the location of σ70 region 4 in different complexes at the galp1 promoter and the effect of the cyclic AMP receptor protein. The positions of DNA cleavage by the reagent are not affected by the cyclic AMP receptor protein. We conclude that transcription activation at the galp1 promoter by the cyclic AMP receptor protein does not involve major conformation changes in or repositioning of σ70 region 4.
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology