Positioning of region 4 of the Escherichia coli RNA polymerase σ70 subunit by a transcription activator

Jonathan A. Bown, Annie Kolb, Claude F. Meares, Akira Ishihama, Stephen D. Minchin, Stephen J W Busby

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

A DNA cleavage reagent, specifically tethered to residue 581 of the Escherichia coli RNA polymerase σ70 subunit, has been used to investigate the location of σ70 region 4 in different complexes at the galp1 promoter and the effect of the cyclic AMP receptor protein. The positions of DNA cleavage by the reagent are not affected by the cyclic AMP receptor protein. We conclude that transcription activation at the galp1 promoter by the cyclic AMP receptor protein does not involve major conformation changes in or repositioning of σ70 region 4.

Original languageEnglish (US)
Pages (from-to)2982-2984
Number of pages3
JournalJournal of Bacteriology
Volume182
Issue number10
DOIs
StatePublished - May 2000

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

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    Bown, J. A., Kolb, A., Meares, C. F., Ishihama, A., Minchin, S. D., & Busby, S. J. W. (2000). Positioning of region 4 of the Escherichia coli RNA polymerase σ70 subunit by a transcription activator. Journal of Bacteriology, 182(10), 2982-2984. https://doi.org/10.1128/JB.182.10.2982-2984.2000