Positioning and guidance of neurons on gold surfaces by directed assembly of proteins using Atomic Force Microscopy

Cristian Staii, Chris Viesselmann, Jason Ballweg, Lifang Shi, Gang-yu Liu, Justin C. Williams, Erik W. Dent, Susan N. Coppersmith, Mark A. Eriksson

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

We demonstrate that Atomic Force Microscopy nanolithography can be used to control effectively the adhesion, growth and interconnectivity of cortical neurons on Au surfaces. We demonstrate immobilization of neurons at well-defined locations on Au surfaces using two different types of patterned proteins: 1) poly-d-lysine (PDL), a positively charged polypeptide used extensively in tissue culture and 2) laminin, a component of the extracellular matrix. Our results show that both PDL and laminin patterns can be used to confine neuronal cells and to control their growth and interconnectivity on Au surfaces, a significant step towards the engineering of artificial neuronal assemblies with well-controlled neuron position and connections.

Original languageEnglish (US)
Pages (from-to)3397-3404
Number of pages8
JournalBiomaterials
Volume30
Issue number20
DOIs
StatePublished - Jul 2009

Fingerprint

Atomic Force Microscopy
Gold
Neurons
Atomic force microscopy
Laminin
Proteins
Lysine
Nanolithography
Tissue culture
Polypeptides
Growth
Immobilization
Extracellular Matrix
Adhesion
Peptides

Keywords

  • AFM
  • Cortical neurons
  • Microfabrication
  • Neural network
  • Protein patterning
  • Self-assembled monolayers

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Positioning and guidance of neurons on gold surfaces by directed assembly of proteins using Atomic Force Microscopy. / Staii, Cristian; Viesselmann, Chris; Ballweg, Jason; Shi, Lifang; Liu, Gang-yu; Williams, Justin C.; Dent, Erik W.; Coppersmith, Susan N.; Eriksson, Mark A.

In: Biomaterials, Vol. 30, No. 20, 07.2009, p. 3397-3404.

Research output: Contribution to journalArticle

Staii, C, Viesselmann, C, Ballweg, J, Shi, L, Liu, G, Williams, JC, Dent, EW, Coppersmith, SN & Eriksson, MA 2009, 'Positioning and guidance of neurons on gold surfaces by directed assembly of proteins using Atomic Force Microscopy', Biomaterials, vol. 30, no. 20, pp. 3397-3404. https://doi.org/10.1016/j.biomaterials.2009.03.027
Staii, Cristian ; Viesselmann, Chris ; Ballweg, Jason ; Shi, Lifang ; Liu, Gang-yu ; Williams, Justin C. ; Dent, Erik W. ; Coppersmith, Susan N. ; Eriksson, Mark A. / Positioning and guidance of neurons on gold surfaces by directed assembly of proteins using Atomic Force Microscopy. In: Biomaterials. 2009 ; Vol. 30, No. 20. pp. 3397-3404.
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