Porcine gonadal and placental isozymes of aromatase cytochrome P450: sub-cellular distribution and support by NADPH-cytochrome P450 reductase

C. J. Corbin, J. M. Trant, Alan J Conley

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Functional differences exist between the porcine gonadal and placental aromatase cytochrome P450 (P450arom) isozymes that are encoded by separate genes. The present experiments investigated the sub-cellular location of these isozymes, their dependence on the redox partner protein NADPH-cytochrome P450 reductase (P450 reductase) for catalytic activity and the release of steroid intermediates during the aromatization of androgens to estrogen. After differential centrifugation, similar levels of gonadal and placental porcine P450arom were found along with P450 reductase in the microsomal compartment using activity and immunoblot analyses. Activity was stimulated much more by recombinant P450 reductase addition, and higher levels of 19-hydroxy and 19-oxo intermediates accumulated during androstenedione and testosterone metabolism, in cells expressing the gonadal compared to the placental isozyme. No other steroid products were identified by HPLC. Thus, the porcine gonadal P450arom is more sensitive to P450 reductase deprivation than is the placental P450arom, accounting in part for catalytic differences between these isozymes.

Original languageEnglish (US)
Pages (from-to)115-124
Number of pages10
JournalMolecular and Cellular Endocrinology
Volume172
Issue number1-2
DOIs
StatePublished - Feb 14 2001

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NADPH-Ferrihemoprotein Reductase
Aromatase
Cytochrome P-450 Enzyme System
Isoenzymes
Oxidoreductases
Swine
Steroids
Aromatization
Centrifugation
Androstenedione
Metabolism
Androgens
Oxidation-Reduction
Testosterone
Catalyst activity
Estrogens
Genes
High Pressure Liquid Chromatography
Cells
Proteins

Keywords

  • Aromatase
  • Cytochrome P450
  • Porcine

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Porcine gonadal and placental isozymes of aromatase cytochrome P450 : sub-cellular distribution and support by NADPH-cytochrome P450 reductase. / Corbin, C. J.; Trant, J. M.; Conley, Alan J.

In: Molecular and Cellular Endocrinology, Vol. 172, No. 1-2, 14.02.2001, p. 115-124.

Research output: Contribution to journalArticle

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