Abstract
Whole blood samples from 211 stray cats from Algiers, Algeria, were cultured to detect the presence of Bartonella species and to evaluate the genetic diversity of B. henselae strains by multiple locus VNTR analysis (MLVA). Bartonella henselae was the only species isolated from 36 (17%) of 211 cats. B. henselae genotype I was the predominant genotype (64%). MLVA typing of 259 strains from 30 bacteremic cats revealed 52 different profiles as compared to only 3 profiles using MLST. Of these 52 profiles, 48 (92.3%) were identified for the first time. One-third of the cats harbored one MLVA profile only. As there was a correlation between the age of cats and the number of MLVA profiles, we hypothesized that the single profile in these cats was the profile of the initial infecting strain. Two-third of the cats harbored 2 to 6 MLVA profiles simultaneously. The similarity of MLVA profiles obtained from the same cat, neighbor-joining clustering and structure-neighbor clustering indicate that such a diversity likely results from two different mechanisms occurring either independently or simultaneously: independent infections and genetic drift from a primary strain.
| Original language | English (US) |
|---|---|
| Article number | e43621 |
| Journal | PLoS One |
| Volume | 7 |
| Issue number | 8 |
| DOIs | |
| State | Published - Aug 27 2012 |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Medicine(all)
Cite this
Population structure of Bartonella henselae in Algerian urban stray cats. / Azzag, Naouelle; Haddad, Nadia; Durand, Benoit; Petit, Elisabeth; Ammouche, Ali; Chomel, Bruno B; Boulouis, Henri Jean.
In: PLoS One, Vol. 7, No. 8, e43621, 27.08.2012.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Population structure of Bartonella henselae in Algerian urban stray cats
AU - Azzag, Naouelle
AU - Haddad, Nadia
AU - Durand, Benoit
AU - Petit, Elisabeth
AU - Ammouche, Ali
AU - Chomel, Bruno B
AU - Boulouis, Henri Jean
PY - 2012/8/27
Y1 - 2012/8/27
N2 - Whole blood samples from 211 stray cats from Algiers, Algeria, were cultured to detect the presence of Bartonella species and to evaluate the genetic diversity of B. henselae strains by multiple locus VNTR analysis (MLVA). Bartonella henselae was the only species isolated from 36 (17%) of 211 cats. B. henselae genotype I was the predominant genotype (64%). MLVA typing of 259 strains from 30 bacteremic cats revealed 52 different profiles as compared to only 3 profiles using MLST. Of these 52 profiles, 48 (92.3%) were identified for the first time. One-third of the cats harbored one MLVA profile only. As there was a correlation between the age of cats and the number of MLVA profiles, we hypothesized that the single profile in these cats was the profile of the initial infecting strain. Two-third of the cats harbored 2 to 6 MLVA profiles simultaneously. The similarity of MLVA profiles obtained from the same cat, neighbor-joining clustering and structure-neighbor clustering indicate that such a diversity likely results from two different mechanisms occurring either independently or simultaneously: independent infections and genetic drift from a primary strain.
AB - Whole blood samples from 211 stray cats from Algiers, Algeria, were cultured to detect the presence of Bartonella species and to evaluate the genetic diversity of B. henselae strains by multiple locus VNTR analysis (MLVA). Bartonella henselae was the only species isolated from 36 (17%) of 211 cats. B. henselae genotype I was the predominant genotype (64%). MLVA typing of 259 strains from 30 bacteremic cats revealed 52 different profiles as compared to only 3 profiles using MLST. Of these 52 profiles, 48 (92.3%) were identified for the first time. One-third of the cats harbored one MLVA profile only. As there was a correlation between the age of cats and the number of MLVA profiles, we hypothesized that the single profile in these cats was the profile of the initial infecting strain. Two-third of the cats harbored 2 to 6 MLVA profiles simultaneously. The similarity of MLVA profiles obtained from the same cat, neighbor-joining clustering and structure-neighbor clustering indicate that such a diversity likely results from two different mechanisms occurring either independently or simultaneously: independent infections and genetic drift from a primary strain.
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UR - http://www.scopus.com/inward/citedby.url?scp=84865404587&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0043621
DO - 10.1371/journal.pone.0043621
M3 - Article
C2 - 22956995
AN - SCOPUS:84865404587
VL - 7
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 8
M1 - e43621
ER -