Population genetic structure of Anopheles arabiensis and Anopheles gambiae in a malaria endemic region of southern Tanzania

Kija R. Ng'Habi, Bart Gj Knols, Yoosook Lee, Heather M. Ferguson, Gregory C Lanzaro

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Genetic diversity is a key factor that enables adaptation and persistence of natural populations towards environmental conditions. It is influenced by the interaction of a natural population's dynamics and the environment it inhabits. Anopheles gambiae s.s. and Anopheles arabiensis are the two major and widespread malaria vectors in sub-Saharan Africa. Several studies have examined the ecology and population dynamics of these vectors. Ecological conditions along the Kilombero valley in Tanzania influence the distribution and population density of these two vector species. It remains unclear whether the ecological diversity within the Kilombero valley has affected the population structure of An. gambiae s.l. populations. The goal of this study was to characterise the genetic structure of sympatric An. gambiae s.s and An. arabiensis populations along the Kilombero valley. Methodology. Mosquitoes were collected from seven locations in Tanzania: six from the Kilombero valley and one outside the valley (∼700 km away) as an out-group. To archive a genome-wide coverage, 13 microsatellite markers from chromosomes X, 2 and 3 were used. Results: High levels of genetic differentiation among An. arabiensis populations was observed, as opposed to An. gambiae s.s., which was genetically undifferentiated across the 6,650 km2 of the Kilombero valley landscape. It appears that genetic differentiation is not attributed to physical barriers or distance, but possibly by ecological diversification within the Kilombero valley. Genetic divergence among An. arabiensis populations (F ST= 0.066) was higher than that of the well-known M and S forms of An. gambiae s. s. in West and Central Africa (F ST= 0.035), suggesting that these populations are maintained by some level of reproductive isolation. Conclusion: It was hypothesized that ecological diversification across the valley may be a driving force for observed An. arabiensis genetic divergence. The impact of the observed An. arabiensis substructure to the prospects for new vector control approaches is discussed.

Original languageEnglish (US)
Article number289
JournalMalaria Journal
Volume10
DOIs
StatePublished - 2011

Fingerprint

Anopheles gambiae
Anopheles
Tanzania
Genetic Structures
Population Genetics
Malaria
Population
Population Dynamics
Reproductive Isolation
Central Africa
Architectural Accessibility
Chromosomes, Human, Pair 3
Western Africa
Chromosomes, Human, Pair 2
Africa South of the Sahara
Population Density
Ecology
Culicidae
Microsatellite Repeats
Genome

ASJC Scopus subject areas

  • Infectious Diseases
  • Parasitology

Cite this

Population genetic structure of Anopheles arabiensis and Anopheles gambiae in a malaria endemic region of southern Tanzania. / Ng'Habi, Kija R.; Knols, Bart Gj; Lee, Yoosook; Ferguson, Heather M.; Lanzaro, Gregory C.

In: Malaria Journal, Vol. 10, 289, 2011.

Research output: Contribution to journalArticle

@article{c6ab9d0a600b4ea0a7ed92f04c85c1f5,
title = "Population genetic structure of Anopheles arabiensis and Anopheles gambiae in a malaria endemic region of southern Tanzania",
abstract = "Background: Genetic diversity is a key factor that enables adaptation and persistence of natural populations towards environmental conditions. It is influenced by the interaction of a natural population's dynamics and the environment it inhabits. Anopheles gambiae s.s. and Anopheles arabiensis are the two major and widespread malaria vectors in sub-Saharan Africa. Several studies have examined the ecology and population dynamics of these vectors. Ecological conditions along the Kilombero valley in Tanzania influence the distribution and population density of these two vector species. It remains unclear whether the ecological diversity within the Kilombero valley has affected the population structure of An. gambiae s.l. populations. The goal of this study was to characterise the genetic structure of sympatric An. gambiae s.s and An. arabiensis populations along the Kilombero valley. Methodology. Mosquitoes were collected from seven locations in Tanzania: six from the Kilombero valley and one outside the valley (∼700 km away) as an out-group. To archive a genome-wide coverage, 13 microsatellite markers from chromosomes X, 2 and 3 were used. Results: High levels of genetic differentiation among An. arabiensis populations was observed, as opposed to An. gambiae s.s., which was genetically undifferentiated across the 6,650 km2 of the Kilombero valley landscape. It appears that genetic differentiation is not attributed to physical barriers or distance, but possibly by ecological diversification within the Kilombero valley. Genetic divergence among An. arabiensis populations (F ST= 0.066) was higher than that of the well-known M and S forms of An. gambiae s. s. in West and Central Africa (F ST= 0.035), suggesting that these populations are maintained by some level of reproductive isolation. Conclusion: It was hypothesized that ecological diversification across the valley may be a driving force for observed An. arabiensis genetic divergence. The impact of the observed An. arabiensis substructure to the prospects for new vector control approaches is discussed.",
author = "Ng'Habi, {Kija R.} and Knols, {Bart Gj} and Yoosook Lee and Ferguson, {Heather M.} and Lanzaro, {Gregory C}",
year = "2011",
doi = "10.1186/1475-2875-10-289",
language = "English (US)",
volume = "10",
journal = "Malaria Journal",
issn = "1475-2875",
publisher = "BioMed Central",

}

TY - JOUR

T1 - Population genetic structure of Anopheles arabiensis and Anopheles gambiae in a malaria endemic region of southern Tanzania

AU - Ng'Habi, Kija R.

AU - Knols, Bart Gj

AU - Lee, Yoosook

AU - Ferguson, Heather M.

AU - Lanzaro, Gregory C

PY - 2011

Y1 - 2011

N2 - Background: Genetic diversity is a key factor that enables adaptation and persistence of natural populations towards environmental conditions. It is influenced by the interaction of a natural population's dynamics and the environment it inhabits. Anopheles gambiae s.s. and Anopheles arabiensis are the two major and widespread malaria vectors in sub-Saharan Africa. Several studies have examined the ecology and population dynamics of these vectors. Ecological conditions along the Kilombero valley in Tanzania influence the distribution and population density of these two vector species. It remains unclear whether the ecological diversity within the Kilombero valley has affected the population structure of An. gambiae s.l. populations. The goal of this study was to characterise the genetic structure of sympatric An. gambiae s.s and An. arabiensis populations along the Kilombero valley. Methodology. Mosquitoes were collected from seven locations in Tanzania: six from the Kilombero valley and one outside the valley (∼700 km away) as an out-group. To archive a genome-wide coverage, 13 microsatellite markers from chromosomes X, 2 and 3 were used. Results: High levels of genetic differentiation among An. arabiensis populations was observed, as opposed to An. gambiae s.s., which was genetically undifferentiated across the 6,650 km2 of the Kilombero valley landscape. It appears that genetic differentiation is not attributed to physical barriers or distance, but possibly by ecological diversification within the Kilombero valley. Genetic divergence among An. arabiensis populations (F ST= 0.066) was higher than that of the well-known M and S forms of An. gambiae s. s. in West and Central Africa (F ST= 0.035), suggesting that these populations are maintained by some level of reproductive isolation. Conclusion: It was hypothesized that ecological diversification across the valley may be a driving force for observed An. arabiensis genetic divergence. The impact of the observed An. arabiensis substructure to the prospects for new vector control approaches is discussed.

AB - Background: Genetic diversity is a key factor that enables adaptation and persistence of natural populations towards environmental conditions. It is influenced by the interaction of a natural population's dynamics and the environment it inhabits. Anopheles gambiae s.s. and Anopheles arabiensis are the two major and widespread malaria vectors in sub-Saharan Africa. Several studies have examined the ecology and population dynamics of these vectors. Ecological conditions along the Kilombero valley in Tanzania influence the distribution and population density of these two vector species. It remains unclear whether the ecological diversity within the Kilombero valley has affected the population structure of An. gambiae s.l. populations. The goal of this study was to characterise the genetic structure of sympatric An. gambiae s.s and An. arabiensis populations along the Kilombero valley. Methodology. Mosquitoes were collected from seven locations in Tanzania: six from the Kilombero valley and one outside the valley (∼700 km away) as an out-group. To archive a genome-wide coverage, 13 microsatellite markers from chromosomes X, 2 and 3 were used. Results: High levels of genetic differentiation among An. arabiensis populations was observed, as opposed to An. gambiae s.s., which was genetically undifferentiated across the 6,650 km2 of the Kilombero valley landscape. It appears that genetic differentiation is not attributed to physical barriers or distance, but possibly by ecological diversification within the Kilombero valley. Genetic divergence among An. arabiensis populations (F ST= 0.066) was higher than that of the well-known M and S forms of An. gambiae s. s. in West and Central Africa (F ST= 0.035), suggesting that these populations are maintained by some level of reproductive isolation. Conclusion: It was hypothesized that ecological diversification across the valley may be a driving force for observed An. arabiensis genetic divergence. The impact of the observed An. arabiensis substructure to the prospects for new vector control approaches is discussed.

UR - http://www.scopus.com/inward/record.url?scp=80053504314&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80053504314&partnerID=8YFLogxK

U2 - 10.1186/1475-2875-10-289

DO - 10.1186/1475-2875-10-289

M3 - Article

C2 - 21975087

AN - SCOPUS:80053504314

VL - 10

JO - Malaria Journal

JF - Malaria Journal

SN - 1475-2875

M1 - 289

ER -