PON1 status does not influence cholinesterase activity in Egyptian agricultural workers exposed to chlorpyrifos

Corie A. Ellison, Alice L. Crane, Matthew R. Bonner, James B. Knaak, Richard W. Browne, Pamela J Lein, James R. Olson

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Animal studies have shown that paraoxonase 1 (PON1) genotype can influence susceptibility to the organophosphorus pesticide chlorpyrifos (CPF). However, Monte Carlo analysis suggests that PON1 genotype may not affect CPF-related toxicity at low exposure conditions in humans. The current study sought to determine the influence of PON1 genotype on the activity of blood cholinesterase as well as the effect of CPF exposure on serum PON1 in workers occupationally exposed to CPF. Saliva, blood and urine were collected from agricultural workers (n=120) from Egypt's Menoufia Governorate to determine PON1 genotype, blood cholinesterase activity, serum PON1 activity towards chlorpyrifos-oxon (CPOase) and paraoxon (POase), and urinary levels of the CPF metabolite 3,5,6-trichloro-2-pyridinol (TCPy). The PON1 55 (P≤0.05) but not the PON1 192 genotype had a significant effect on CPOase activity. However, both the PON1 55 (P≤0.05) and PON1 192 (P≤0.001) genotypes had a significant effect on POase activity. Workers had significantly inhibited AChE and BuChE after CPF application; however, neither CPOase activity nor POase activity was associated with ChE depression when adjusted for CPF exposure (as determined by urinary TCPy levels) and stratified by PON1 genotype. CPOase and POase activity were also generally unaffected by CPF exposure although there were alterations in activity within specific genotype groups. Together, these results suggest that workers retained the capacity to detoxify chlorpyrifos-oxon under the exposure conditions experienced by this study population regardless of PON1 genotype and activity and that effects of CPF exposure on PON1 activity are minimal.

Original languageEnglish (US)
Pages (from-to)308-315
Number of pages8
JournalToxicology and Applied Pharmacology
Volume265
Issue number3
DOIs
StatePublished - Dec 15 2012

Fingerprint

Aryldialkylphosphatase
Chlorpyrifos
Cholinesterases
Genotype
Blood
Farmers
Paraoxon
Egypt
Metabolites
Serum
Saliva
Pesticides
Toxicity

Keywords

  • Acetylcholinesterase
  • Biomarker
  • Butyrylcholinesterase
  • Chlorpyrifos
  • Organophosphorus
  • Paraoxonase 1

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

PON1 status does not influence cholinesterase activity in Egyptian agricultural workers exposed to chlorpyrifos. / Ellison, Corie A.; Crane, Alice L.; Bonner, Matthew R.; Knaak, James B.; Browne, Richard W.; Lein, Pamela J; Olson, James R.

In: Toxicology and Applied Pharmacology, Vol. 265, No. 3, 15.12.2012, p. 308-315.

Research output: Contribution to journalArticle

Ellison, Corie A. ; Crane, Alice L. ; Bonner, Matthew R. ; Knaak, James B. ; Browne, Richard W. ; Lein, Pamela J ; Olson, James R. / PON1 status does not influence cholinesterase activity in Egyptian agricultural workers exposed to chlorpyrifos. In: Toxicology and Applied Pharmacology. 2012 ; Vol. 265, No. 3. pp. 308-315.
@article{c38a5d64e6584b0b963b141118f1417a,
title = "PON1 status does not influence cholinesterase activity in Egyptian agricultural workers exposed to chlorpyrifos",
abstract = "Animal studies have shown that paraoxonase 1 (PON1) genotype can influence susceptibility to the organophosphorus pesticide chlorpyrifos (CPF). However, Monte Carlo analysis suggests that PON1 genotype may not affect CPF-related toxicity at low exposure conditions in humans. The current study sought to determine the influence of PON1 genotype on the activity of blood cholinesterase as well as the effect of CPF exposure on serum PON1 in workers occupationally exposed to CPF. Saliva, blood and urine were collected from agricultural workers (n=120) from Egypt's Menoufia Governorate to determine PON1 genotype, blood cholinesterase activity, serum PON1 activity towards chlorpyrifos-oxon (CPOase) and paraoxon (POase), and urinary levels of the CPF metabolite 3,5,6-trichloro-2-pyridinol (TCPy). The PON1 55 (P≤0.05) but not the PON1 192 genotype had a significant effect on CPOase activity. However, both the PON1 55 (P≤0.05) and PON1 192 (P≤0.001) genotypes had a significant effect on POase activity. Workers had significantly inhibited AChE and BuChE after CPF application; however, neither CPOase activity nor POase activity was associated with ChE depression when adjusted for CPF exposure (as determined by urinary TCPy levels) and stratified by PON1 genotype. CPOase and POase activity were also generally unaffected by CPF exposure although there were alterations in activity within specific genotype groups. Together, these results suggest that workers retained the capacity to detoxify chlorpyrifos-oxon under the exposure conditions experienced by this study population regardless of PON1 genotype and activity and that effects of CPF exposure on PON1 activity are minimal.",
keywords = "Acetylcholinesterase, Biomarker, Butyrylcholinesterase, Chlorpyrifos, Organophosphorus, Paraoxonase 1",
author = "Ellison, {Corie A.} and Crane, {Alice L.} and Bonner, {Matthew R.} and Knaak, {James B.} and Browne, {Richard W.} and Lein, {Pamela J} and Olson, {James R.}",
year = "2012",
month = "12",
day = "15",
doi = "10.1016/j.taap.2012.08.031",
language = "English (US)",
volume = "265",
pages = "308--315",
journal = "Toxicology and Applied Pharmacology",
issn = "0041-008X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - PON1 status does not influence cholinesterase activity in Egyptian agricultural workers exposed to chlorpyrifos

AU - Ellison, Corie A.

AU - Crane, Alice L.

AU - Bonner, Matthew R.

AU - Knaak, James B.

AU - Browne, Richard W.

AU - Lein, Pamela J

AU - Olson, James R.

PY - 2012/12/15

Y1 - 2012/12/15

N2 - Animal studies have shown that paraoxonase 1 (PON1) genotype can influence susceptibility to the organophosphorus pesticide chlorpyrifos (CPF). However, Monte Carlo analysis suggests that PON1 genotype may not affect CPF-related toxicity at low exposure conditions in humans. The current study sought to determine the influence of PON1 genotype on the activity of blood cholinesterase as well as the effect of CPF exposure on serum PON1 in workers occupationally exposed to CPF. Saliva, blood and urine were collected from agricultural workers (n=120) from Egypt's Menoufia Governorate to determine PON1 genotype, blood cholinesterase activity, serum PON1 activity towards chlorpyrifos-oxon (CPOase) and paraoxon (POase), and urinary levels of the CPF metabolite 3,5,6-trichloro-2-pyridinol (TCPy). The PON1 55 (P≤0.05) but not the PON1 192 genotype had a significant effect on CPOase activity. However, both the PON1 55 (P≤0.05) and PON1 192 (P≤0.001) genotypes had a significant effect on POase activity. Workers had significantly inhibited AChE and BuChE after CPF application; however, neither CPOase activity nor POase activity was associated with ChE depression when adjusted for CPF exposure (as determined by urinary TCPy levels) and stratified by PON1 genotype. CPOase and POase activity were also generally unaffected by CPF exposure although there were alterations in activity within specific genotype groups. Together, these results suggest that workers retained the capacity to detoxify chlorpyrifos-oxon under the exposure conditions experienced by this study population regardless of PON1 genotype and activity and that effects of CPF exposure on PON1 activity are minimal.

AB - Animal studies have shown that paraoxonase 1 (PON1) genotype can influence susceptibility to the organophosphorus pesticide chlorpyrifos (CPF). However, Monte Carlo analysis suggests that PON1 genotype may not affect CPF-related toxicity at low exposure conditions in humans. The current study sought to determine the influence of PON1 genotype on the activity of blood cholinesterase as well as the effect of CPF exposure on serum PON1 in workers occupationally exposed to CPF. Saliva, blood and urine were collected from agricultural workers (n=120) from Egypt's Menoufia Governorate to determine PON1 genotype, blood cholinesterase activity, serum PON1 activity towards chlorpyrifos-oxon (CPOase) and paraoxon (POase), and urinary levels of the CPF metabolite 3,5,6-trichloro-2-pyridinol (TCPy). The PON1 55 (P≤0.05) but not the PON1 192 genotype had a significant effect on CPOase activity. However, both the PON1 55 (P≤0.05) and PON1 192 (P≤0.001) genotypes had a significant effect on POase activity. Workers had significantly inhibited AChE and BuChE after CPF application; however, neither CPOase activity nor POase activity was associated with ChE depression when adjusted for CPF exposure (as determined by urinary TCPy levels) and stratified by PON1 genotype. CPOase and POase activity were also generally unaffected by CPF exposure although there were alterations in activity within specific genotype groups. Together, these results suggest that workers retained the capacity to detoxify chlorpyrifos-oxon under the exposure conditions experienced by this study population regardless of PON1 genotype and activity and that effects of CPF exposure on PON1 activity are minimal.

KW - Acetylcholinesterase

KW - Biomarker

KW - Butyrylcholinesterase

KW - Chlorpyrifos

KW - Organophosphorus

KW - Paraoxonase 1

UR - http://www.scopus.com/inward/record.url?scp=84870302200&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84870302200&partnerID=8YFLogxK

U2 - 10.1016/j.taap.2012.08.031

DO - 10.1016/j.taap.2012.08.031

M3 - Article

VL - 265

SP - 308

EP - 315

JO - Toxicology and Applied Pharmacology

JF - Toxicology and Applied Pharmacology

SN - 0041-008X

IS - 3

ER -