Abstract
Background: In the fission yeast Schizosaccharomyces pombe, cell growth takes place exclusively at both ends of the cylindrical cell. During this highly polarized growth, microtubules are responsible for the placement of the cell-end marker proteins, the Tea1-Tea4/Wsh3 complex, which recruits the Pom1 DYRK-family protein kinase. Pom1 is required for proper positioning of growth sites, and the Δpom1 mutation brings about monopolar cell growth. Results: Pom1 kinase physically interacts with Rga4, which has a GAP (GTPase-activating protein) domain for Rho-family GTPase. Genetic and biochemical evidence indicates that Rga4 functions as GAP for the Cdc42 GTPase, an evolutionarily conserved regulator of F-actin. CRIB (Cdc42/Rac interactive binding)-GFP microscopy has revealed that GTP-bound, active Cdc42 is concentrated to growing cell ends accompanied by developed F-actin structures, where the Rga4 GAP is excluded. The monopolar Δpom1 mutant fails to eliminate Rga4 from the nongrowing cell end, resulting in monopolar distribution of GTP-Cdc42 to the growing cell end. However, mutational inactivation of Rga4 allows Cdc42 to be active at both ends of Δpom1 cells, suggesting that mislocalization of Rga4 in the Δpom1 mutant contributes to its monopolar phenotype. Conclusions: Pom1 kinase recruited to cell ends by the Tea1-Tea4/Wsh3 complex is essential for proper localization of a GAP for Cdc42, Rga4, which ensures bipolar localization of GTP-bound, active Cdc42. Because of the established role of Cdc42 in F-actin formation, these observations provide a new insight into how the microtubule system achieves localized formation of F-actin to generate cell polarity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 322-330 |
| Number of pages | 9 |
| Journal | Current Biology |
| Volume | 18 |
| Issue number | 5 |
| DOIs | |
| State | Published - Mar 11 2008 |
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Keywords
- CELLBIO
- CELLCYCLE
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
Cite this
Pom1 DYRK Regulates Localization of the Rga4 GAP to Ensure Bipolar Activation of Cdc42 in Fission Yeast. / Tatebe, Hisashi; Nakano, Kentaro; Maximo, Rachel; Shiozaki, Kazuhiro.
In: Current Biology, Vol. 18, No. 5, 11.03.2008, p. 322-330.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Pom1 DYRK Regulates Localization of the Rga4 GAP to Ensure Bipolar Activation of Cdc42 in Fission Yeast
AU - Tatebe, Hisashi
AU - Nakano, Kentaro
AU - Maximo, Rachel
AU - Shiozaki, Kazuhiro
PY - 2008/3/11
Y1 - 2008/3/11
N2 - Background: In the fission yeast Schizosaccharomyces pombe, cell growth takes place exclusively at both ends of the cylindrical cell. During this highly polarized growth, microtubules are responsible for the placement of the cell-end marker proteins, the Tea1-Tea4/Wsh3 complex, which recruits the Pom1 DYRK-family protein kinase. Pom1 is required for proper positioning of growth sites, and the Δpom1 mutation brings about monopolar cell growth. Results: Pom1 kinase physically interacts with Rga4, which has a GAP (GTPase-activating protein) domain for Rho-family GTPase. Genetic and biochemical evidence indicates that Rga4 functions as GAP for the Cdc42 GTPase, an evolutionarily conserved regulator of F-actin. CRIB (Cdc42/Rac interactive binding)-GFP microscopy has revealed that GTP-bound, active Cdc42 is concentrated to growing cell ends accompanied by developed F-actin structures, where the Rga4 GAP is excluded. The monopolar Δpom1 mutant fails to eliminate Rga4 from the nongrowing cell end, resulting in monopolar distribution of GTP-Cdc42 to the growing cell end. However, mutational inactivation of Rga4 allows Cdc42 to be active at both ends of Δpom1 cells, suggesting that mislocalization of Rga4 in the Δpom1 mutant contributes to its monopolar phenotype. Conclusions: Pom1 kinase recruited to cell ends by the Tea1-Tea4/Wsh3 complex is essential for proper localization of a GAP for Cdc42, Rga4, which ensures bipolar localization of GTP-bound, active Cdc42. Because of the established role of Cdc42 in F-actin formation, these observations provide a new insight into how the microtubule system achieves localized formation of F-actin to generate cell polarity.
AB - Background: In the fission yeast Schizosaccharomyces pombe, cell growth takes place exclusively at both ends of the cylindrical cell. During this highly polarized growth, microtubules are responsible for the placement of the cell-end marker proteins, the Tea1-Tea4/Wsh3 complex, which recruits the Pom1 DYRK-family protein kinase. Pom1 is required for proper positioning of growth sites, and the Δpom1 mutation brings about monopolar cell growth. Results: Pom1 kinase physically interacts with Rga4, which has a GAP (GTPase-activating protein) domain for Rho-family GTPase. Genetic and biochemical evidence indicates that Rga4 functions as GAP for the Cdc42 GTPase, an evolutionarily conserved regulator of F-actin. CRIB (Cdc42/Rac interactive binding)-GFP microscopy has revealed that GTP-bound, active Cdc42 is concentrated to growing cell ends accompanied by developed F-actin structures, where the Rga4 GAP is excluded. The monopolar Δpom1 mutant fails to eliminate Rga4 from the nongrowing cell end, resulting in monopolar distribution of GTP-Cdc42 to the growing cell end. However, mutational inactivation of Rga4 allows Cdc42 to be active at both ends of Δpom1 cells, suggesting that mislocalization of Rga4 in the Δpom1 mutant contributes to its monopolar phenotype. Conclusions: Pom1 kinase recruited to cell ends by the Tea1-Tea4/Wsh3 complex is essential for proper localization of a GAP for Cdc42, Rga4, which ensures bipolar localization of GTP-bound, active Cdc42. Because of the established role of Cdc42 in F-actin formation, these observations provide a new insight into how the microtubule system achieves localized formation of F-actin to generate cell polarity.
KW - CELLBIO
KW - CELLCYCLE
UR - http://www.scopus.com/inward/record.url?scp=40149109750&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=40149109750&partnerID=8YFLogxK
U2 - 10.1016/j.cub.2008.02.005
DO - 10.1016/j.cub.2008.02.005
M3 - Article
VL - 18
SP - 322
EP - 330
JO - Current Biology
JF - Current Biology
SN - 0960-9822
IS - 5
ER -