Abstract
This paper presents the initial effort in anti-HIV infection using glycosphingolipid-based nanostructures. HIV infection of CD4 negative cells is initiated by the binding of the viral envelope glycoprotein gp120 to galactosylceramide (GalCer), a glycosphingolipid that serves as the cellular receptor for viral recognition. A series of nanostructures of GalCer are designed and produced using an AFM-based lithography method known as nanografting. The geometry dependence of recombinant gp120 binding to these nanostructures is monitored using high-resolution AFM imaging. Gp120 molecules are found to favor binding sites that allow for polyvalent interactions. Increased adsorption at the intersection of two lines, or between two parallel lines with matching separation for trimeric binding, strongly suggests that trivalent interactions are dominant in gp120-GalCer nanostructure interactions. Systematic distance-dependence studies, using parallel nanolines with various separations, reveal a separation of 4.8 nm, matching the separation of V3 loops in gp120 trimers. This investigation demonstrates that nanotechnology provides a powerful tool for investigating and guiding polyvalent interactions among biological systems.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 201-210 |
| Number of pages | 10 |
| Journal | Nanobiotechnology |
| Volume | 1 |
| Issue number | 2 |
| DOIs | |
| State | Published - 2005 |
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Keywords
- Atomic force microscopy
- Envelope protein gp120
- HIV-1
- Nanofabrication
- Polyvalent interactions
- Self-assembled monolayers
ASJC Scopus subject areas
- Biotechnology
- Molecular Biology
- Molecular Medicine
Cite this
Polyvalent interactions of HIV-gp120 protein and nanostructures of carbohydrate ligands. / Yu, Jing Jiang; Nolting, Birte; Yih, Horng Tan; Li, Xue; Gervay-Hague, Jacquelyn; Liu, Gang-yu.
In: Nanobiotechnology, Vol. 1, No. 2, 2005, p. 201-210.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Polyvalent interactions of HIV-gp120 protein and nanostructures of carbohydrate ligands
AU - Yu, Jing Jiang
AU - Nolting, Birte
AU - Yih, Horng Tan
AU - Li, Xue
AU - Gervay-Hague, Jacquelyn
AU - Liu, Gang-yu
PY - 2005
Y1 - 2005
N2 - This paper presents the initial effort in anti-HIV infection using glycosphingolipid-based nanostructures. HIV infection of CD4 negative cells is initiated by the binding of the viral envelope glycoprotein gp120 to galactosylceramide (GalCer), a glycosphingolipid that serves as the cellular receptor for viral recognition. A series of nanostructures of GalCer are designed and produced using an AFM-based lithography method known as nanografting. The geometry dependence of recombinant gp120 binding to these nanostructures is monitored using high-resolution AFM imaging. Gp120 molecules are found to favor binding sites that allow for polyvalent interactions. Increased adsorption at the intersection of two lines, or between two parallel lines with matching separation for trimeric binding, strongly suggests that trivalent interactions are dominant in gp120-GalCer nanostructure interactions. Systematic distance-dependence studies, using parallel nanolines with various separations, reveal a separation of 4.8 nm, matching the separation of V3 loops in gp120 trimers. This investigation demonstrates that nanotechnology provides a powerful tool for investigating and guiding polyvalent interactions among biological systems.
AB - This paper presents the initial effort in anti-HIV infection using glycosphingolipid-based nanostructures. HIV infection of CD4 negative cells is initiated by the binding of the viral envelope glycoprotein gp120 to galactosylceramide (GalCer), a glycosphingolipid that serves as the cellular receptor for viral recognition. A series of nanostructures of GalCer are designed and produced using an AFM-based lithography method known as nanografting. The geometry dependence of recombinant gp120 binding to these nanostructures is monitored using high-resolution AFM imaging. Gp120 molecules are found to favor binding sites that allow for polyvalent interactions. Increased adsorption at the intersection of two lines, or between two parallel lines with matching separation for trimeric binding, strongly suggests that trivalent interactions are dominant in gp120-GalCer nanostructure interactions. Systematic distance-dependence studies, using parallel nanolines with various separations, reveal a separation of 4.8 nm, matching the separation of V3 loops in gp120 trimers. This investigation demonstrates that nanotechnology provides a powerful tool for investigating and guiding polyvalent interactions among biological systems.
KW - Atomic force microscopy
KW - Envelope protein gp120
KW - HIV-1
KW - Nanofabrication
KW - Polyvalent interactions
KW - Self-assembled monolayers
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UR - http://www.scopus.com/inward/citedby.url?scp=33646885930&partnerID=8YFLogxK
U2 - 10.1385/NBT:1:2:201
DO - 10.1385/NBT:1:2:201
M3 - Article
AN - SCOPUS:33646885930
VL - 1
SP - 201
EP - 210
JO - Nanobiotechnology
JF - Nanobiotechnology
SN - 1551-1286
IS - 2
ER -