Polyubiquitination of APOBEC3G is essential for its degradation by HIV-1 Vif

Qiujia Shao, Yudi Wang, James Hildreth, Bindong Liu

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Proteasomal degradation of APOBEC3G is a critical step for human immunodeficiency virus type 1 (HIV-1) replication. However, the necessity for polyubiquitination of APOBEC3G in this process is still controversial. In this study, we showed that although macaque simian immunodeficiency virus (SIVmac) Vif is more stable than HIV-1 Vif in human cells, SIVmac Vif induces degradation of APBOEC3G as efficiently as HIV-1 Vif. Overexpression of APOBEC3G or lysine-free APOBEC3G stabilized HIV-1 Vif, indicating that APOBEC3G degradation is independent of the degradation of Vif. Furthermore, an in vivo polyubiquitination assay showed that lysine-free APOBEC3G was also polyubiquitinated. These data suggest that polyubiquitination of APOBEC3G, not that of HIV-1 Vif, is crucial for APOBEC3G degradation.

Original languageEnglish (US)
Pages (from-to)4840-4844
Number of pages5
JournalJournal of Virology
Volume84
Issue number9
DOIs
StatePublished - May 2010
Externally publishedYes

ASJC Scopus subject areas

  • Immunology
  • Virology

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