Polyphosphate kinase protects Salmonella enterica from weak organic acid stress

Marian Price-Carter, Thomas G. Fazzio, Ester Ibañez Vallbona, John R. Roth

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Mutants of Salmonella enterica lacking polyphosphate kinase (ppk) grow poorly in the presence of the weak organic acids acetate, propionate, and benzoate. This sensitivity is corrected by methionine and seems to result from destabilization of MetA (homoserine transsuccinylase), the first enzyme in methionine biosynthesis. The MetA protein is known to be sensitive to thermal inactivation, and ppk mutants are more sensitive to heat-induced methionine auxotrophy. Peroxide increases the sensitivity of ppk mutants to both heat and acid and may oxidatively damage (carbonylate) destabilized MetA. While acid appears to impair methionine biosynthesis, it leads to derepression of MetA and may inhibit growth by causing toxic accumulation of denatured protein. This is supported by the observation that the overexpression of MetA in ppk mutants causes acid sensitivity that is not corrected by methionine. We propose that polyphosphate acts as a chemical chaperone that helps refold MetA and/or may stimulate proteolysis of toxic denatured protein. The instability of MetA protein may provide a metabolic fuse that blocks growth under conditions that denature proteins; the sensitivity of this fuse is modulated by polyphosphate.

Original languageEnglish (US)
Pages (from-to)3088-3099
Number of pages12
JournalJournal of Bacteriology
Volume187
Issue number9
DOIs
StatePublished - May 2005

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Salmonella enterica
Methionine
Acids
Polyphosphates
Hot Temperature
Poisons
Proteins
Homoserine O-Succinyltransferase
Benzoates
Peroxides
Propionates
Growth
Proteolysis
Acetates
polyphosphate kinase
Enzymes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Polyphosphate kinase protects Salmonella enterica from weak organic acid stress. / Price-Carter, Marian; Fazzio, Thomas G.; Vallbona, Ester Ibañez; Roth, John R.

In: Journal of Bacteriology, Vol. 187, No. 9, 05.2005, p. 3088-3099.

Research output: Contribution to journalArticle

Price-Carter, Marian ; Fazzio, Thomas G. ; Vallbona, Ester Ibañez ; Roth, John R. / Polyphosphate kinase protects Salmonella enterica from weak organic acid stress. In: Journal of Bacteriology. 2005 ; Vol. 187, No. 9. pp. 3088-3099.
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