Polynucleotide phosphorylase independently controls virulence factor expression levels and export in Yersinia spp.

Jason A. Rosenzweig, Brett Chromy, Andrea Echeverry, Jing Yang, Becky Adkins, Gregory V. Plano, Sandra McCutchen-Maloney, Kurt Schesser

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Previously, it was shown that optimal functioning of the Yersinia type III secretion system (T3SS) in cell culture infection assays requires the exoribonuclease polynucleotide phosphorylase (PNPase) and that normal T3SS activity could be restored in the Δpnp strains by expressing just the ∼70-aa S1 RNA-binding domain of PNPase. Here, it is shown that the Yersinia Δpnp strain is less virulent in the mouse compared with the isogenic wild-type strain. To begin to understand what could be limiting T3SS activity in the absence of PNPase, T3SS-encoding transcripts and proteins in the Yersinia Δpnp strains were analyzed. Surprisingly, it was found that the Δpnp Yersinia strains possessed enhanced levels of T3SS-encoding transcripts and proteins compared with the wild-type strains. We then found that an S1 variant containing a disruption in its RNA-binding subdomain was inactive in terms of restoring normal T3SS activity. However, T3SS expression levels did not differ between Δpnp strains expressing active and inactive S1 proteins, further showing that T3SS activity and expression levels, at least as related to PNPase and its S1 domain, are not linked. The results suggest that PNPase affects the expression and activity of the T3SS by distinct mechanisms and that the S1-dependent effect on T3SS activity involves an RNA intermediate.

Original languageEnglish (US)
Pages (from-to)255-264
Number of pages10
JournalFEMS Microbiology Letters
Volume270
Issue number2
DOIs
StatePublished - May 2007
Externally publishedYes

Keywords

  • PNPase
  • Secretion
  • Yersinia
  • Yops

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

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    Rosenzweig, J. A., Chromy, B., Echeverry, A., Yang, J., Adkins, B., Plano, G. V., McCutchen-Maloney, S., & Schesser, K. (2007). Polynucleotide phosphorylase independently controls virulence factor expression levels and export in Yersinia spp. FEMS Microbiology Letters, 270(2), 255-264. https://doi.org/10.1111/j.1574-6968.2007.00689.x