Polymorphism in soluble epoxide hydrolase and blood pressure in spontaneously hypertensive rats

Myriam Fornage, Cruz A. Hinojos, Barbara W. Nurowska, Eric Boerwinkle, Bruce D. Hammock, Christophe H P Morisseau, Peter A. Doris

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

We measured soluble epoxide hydrolase (sEH) renal gene expression in prehypertensive (4 to 5 weeks old) spontaneously hypertensive rats of the Heidelberg SP substrain (SHR [Heid]) and when blood pressure levels entered the hypertensive plateau (17 to 18 weeks old) and compared expression with matched Wistar-Kyoto (WKY [Heid]) rats. Less expression of the gene encoding sEH (EPHX2) was observed in SHR (Heid) than in WKY (Heid). Analysis of sEH protein abundance showed a similar difference. However, no correlation between sEH abundance and blood pressure was observed in the F2 progeny of a parental strain cross. Measurement of protein abundance in SHR and WKY obtained from Charles River confirmed a recent report that abundance of sEH was greater in SHR (CRiv) than WKY (CRiv) strains. Polymorphisms were detected in EPHX2. Resequencing revealed that 2 alleles of EPHX2 exist in these 4 rat strains, differing by 4 single nucleotide polymorphisms, of which 3 produce nonsynonymous amino acid substitutions. The ancestral allele was shared by SHR (Heid) and WKY (CRiv), and the variant allele was shared by WKY (Heid) and SHR (CRiv). Activity of sEH was greater in animals carrying the variant allele. However, inheritance of this allele was not correlated with blood pressure in the F2 progeny of a cross between SHR (Heid) and WKY (Heid). These data indicate that sequence variation determining functional alterations in EPHX2 is not likely to contribute to blood pressure levels in SHR.

Original languageEnglish (US)
Pages (from-to)485-490
Number of pages6
JournalHypertension
Volume40
Issue number4
DOIs
StatePublished - Oct 1 2002

Fingerprint

Epoxide Hydrolases
Inbred SHR Rats
Blood Pressure
Alleles
Gene Expression
Inbred WKY Rats
Amino Acid Substitution
Rivers
Single Nucleotide Polymorphism
Proteins
Kidney

Keywords

  • Genetics
  • Hypertension, genetic
  • Metabolism
  • Polymorphism
  • Rats, inbred SHR

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Fornage, M., Hinojos, C. A., Nurowska, B. W., Boerwinkle, E., Hammock, B. D., Morisseau, C. H. P., & Doris, P. A. (2002). Polymorphism in soluble epoxide hydrolase and blood pressure in spontaneously hypertensive rats. Hypertension, 40(4), 485-490. https://doi.org/10.1161/01.HYP.0000032278.75806.68

Polymorphism in soluble epoxide hydrolase and blood pressure in spontaneously hypertensive rats. / Fornage, Myriam; Hinojos, Cruz A.; Nurowska, Barbara W.; Boerwinkle, Eric; Hammock, Bruce D.; Morisseau, Christophe H P; Doris, Peter A.

In: Hypertension, Vol. 40, No. 4, 01.10.2002, p. 485-490.

Research output: Contribution to journalArticle

Fornage, M, Hinojos, CA, Nurowska, BW, Boerwinkle, E, Hammock, BD, Morisseau, CHP & Doris, PA 2002, 'Polymorphism in soluble epoxide hydrolase and blood pressure in spontaneously hypertensive rats', Hypertension, vol. 40, no. 4, pp. 485-490. https://doi.org/10.1161/01.HYP.0000032278.75806.68
Fornage M, Hinojos CA, Nurowska BW, Boerwinkle E, Hammock BD, Morisseau CHP et al. Polymorphism in soluble epoxide hydrolase and blood pressure in spontaneously hypertensive rats. Hypertension. 2002 Oct 1;40(4):485-490. https://doi.org/10.1161/01.HYP.0000032278.75806.68
Fornage, Myriam ; Hinojos, Cruz A. ; Nurowska, Barbara W. ; Boerwinkle, Eric ; Hammock, Bruce D. ; Morisseau, Christophe H P ; Doris, Peter A. / Polymorphism in soluble epoxide hydrolase and blood pressure in spontaneously hypertensive rats. In: Hypertension. 2002 ; Vol. 40, No. 4. pp. 485-490.
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