Polymeric multilayers that contain silver nanoparticles can be stamped onto biological tissues to provide antibacterial activity

Ankit Agarwal, Kathleen M. Guthrie, Charles J. Czuprynski, Michael J. Schurr, Jonathan F. McAnulty, Christopher J Murphy, Nicholas L. Abbott

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The design of polyelectrolyte multilayers (PEMs) that can be prefabricated on an elastomeric stamp and mechanically transferred onto biomedically-relevant soft materials, including medical-grade silicone elastomers (E'∼450-1500 kPa; E'-elastic modulus) and the dermis of cadaver skin (E'∼200-600 kPa), is reported. Whereas initial attempts to stamp PEMs formed from poly(allylamine hydrochloride) and poly(acrylic acid) resulted in minimal transfer onto soft materials, we report that integration of micrometer-sized beads into the PEMs (thicknesses of 6-160 nm) led to their quantitative transfer within 30 seconds of contact at a pressure of ∼196 kPa. To demonstrate the utility of this approach, PEMs were impregnated with a range of loadings of silver-nanoparticles and stamped onto the dermis of human cadaver skin (a wound-simulant) that was subsequently incubated with bacterial cultures. Skin dermis stamped with PEMs that released 0.25 ± 0.01 μg cm-2 of silver ions caused a 6 log10 reduction in colony forming units of Staphylococcus epidermidis and Pseudomonas aeruginosa within 12 h. Significantly, this level of silver release is below that which is cytotoxic to NIH 3T3 mouse fibroblast cells. Overall, this study describes a general and facile approach for the functionalization of biomaterial surfaces without subjecting them to potentially deleterious processing conditions.

Original languageEnglish (US)
Pages (from-to)1863-1873
Number of pages11
JournalAdvanced Functional Materials
Volume21
Issue number10
DOIs
StatePublished - May 24 2011

Fingerprint

Polyelectrolytes
Silver
Multilayers
silver
Tissue
Nanoparticles
nanoparticles
Skin
pseudomonas
staphylococcus
fibroblasts
hydrochlorides
acrylic acid
silicones
elastomers
beads
mice
micrometers
grade
modulus of elasticity

Keywords

  • microparticles
  • Polymer multilayers
  • silver-nanoparticles
  • stamping
  • wounds

ASJC Scopus subject areas

  • Biomaterials
  • Electrochemistry
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Polymeric multilayers that contain silver nanoparticles can be stamped onto biological tissues to provide antibacterial activity. / Agarwal, Ankit; Guthrie, Kathleen M.; Czuprynski, Charles J.; Schurr, Michael J.; McAnulty, Jonathan F.; Murphy, Christopher J; Abbott, Nicholas L.

In: Advanced Functional Materials, Vol. 21, No. 10, 24.05.2011, p. 1863-1873.

Research output: Contribution to journalArticle

Agarwal, Ankit ; Guthrie, Kathleen M. ; Czuprynski, Charles J. ; Schurr, Michael J. ; McAnulty, Jonathan F. ; Murphy, Christopher J ; Abbott, Nicholas L. / Polymeric multilayers that contain silver nanoparticles can be stamped onto biological tissues to provide antibacterial activity. In: Advanced Functional Materials. 2011 ; Vol. 21, No. 10. pp. 1863-1873.
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