Abstract
Objective: Toxigenic Clostridium difficile, the etiologic agent of C. difficile-associated diarrhea (CDAD), is the most common cause of hospital- acquired diarrhea in many developed countries. In spite of the endemic nature of diarrhea in China, few reports of C. difficile infection in this country have been documented in the literature. Methods: In this study, a polymerase chain reaction (PCR) assay was used to determine the presence of toxigenic C. difficile in the stools of 19 patients who developed diarrhea after antibiotic use in a hospital in China. Clostridium difficile was also cultured from the stool of these patients. After DNA extraction, PCR was performed with primers targeting C. difficile toxin A and toxin B gene sequences. Results: Toxin sequences were detected in 5 of 19 (26%) specimens analyzed. To determine strain identity, these isolates were genotyped using arbitrarily primed PCR (AP-PCR). Three AP-PCR profiles were identified among these isolates. Three of these isolates had the same DNA bending pattern, and they were isolated from patients who were at the same institution. Conclusions: These preliminary results indicate that C. difficile may be involved in some diarrhea cases in China. This study may aid in the understanding of the nature of endemic diarrhea in this country.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 85-87 |
| Number of pages | 3 |
| Journal | International Journal of Infectious Diseases |
| Volume | 2 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1997 |
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Keywords
- China
- Clostridium difficile
- Diarrhea
ASJC Scopus subject areas
- Microbiology (medical)
Cite this
Polymerase chain reaction for identification and typing of Clostridium difficile isolated from Chinese patients. / Liu, Yan Lei; Lu, Xin Hua; Tang, Yajarayma J.; Lam, Kan; Silva, Joseph; Leung, Joseph.
In: International Journal of Infectious Diseases, Vol. 2, No. 2, 1997, p. 85-87.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Polymerase chain reaction for identification and typing of Clostridium difficile isolated from Chinese patients
AU - Liu, Yan Lei
AU - Lu, Xin Hua
AU - Tang, Yajarayma J.
AU - Lam, Kan
AU - Silva, Joseph
AU - Leung, Joseph
PY - 1997
Y1 - 1997
N2 - Objective: Toxigenic Clostridium difficile, the etiologic agent of C. difficile-associated diarrhea (CDAD), is the most common cause of hospital- acquired diarrhea in many developed countries. In spite of the endemic nature of diarrhea in China, few reports of C. difficile infection in this country have been documented in the literature. Methods: In this study, a polymerase chain reaction (PCR) assay was used to determine the presence of toxigenic C. difficile in the stools of 19 patients who developed diarrhea after antibiotic use in a hospital in China. Clostridium difficile was also cultured from the stool of these patients. After DNA extraction, PCR was performed with primers targeting C. difficile toxin A and toxin B gene sequences. Results: Toxin sequences were detected in 5 of 19 (26%) specimens analyzed. To determine strain identity, these isolates were genotyped using arbitrarily primed PCR (AP-PCR). Three AP-PCR profiles were identified among these isolates. Three of these isolates had the same DNA bending pattern, and they were isolated from patients who were at the same institution. Conclusions: These preliminary results indicate that C. difficile may be involved in some diarrhea cases in China. This study may aid in the understanding of the nature of endemic diarrhea in this country.
AB - Objective: Toxigenic Clostridium difficile, the etiologic agent of C. difficile-associated diarrhea (CDAD), is the most common cause of hospital- acquired diarrhea in many developed countries. In spite of the endemic nature of diarrhea in China, few reports of C. difficile infection in this country have been documented in the literature. Methods: In this study, a polymerase chain reaction (PCR) assay was used to determine the presence of toxigenic C. difficile in the stools of 19 patients who developed diarrhea after antibiotic use in a hospital in China. Clostridium difficile was also cultured from the stool of these patients. After DNA extraction, PCR was performed with primers targeting C. difficile toxin A and toxin B gene sequences. Results: Toxin sequences were detected in 5 of 19 (26%) specimens analyzed. To determine strain identity, these isolates were genotyped using arbitrarily primed PCR (AP-PCR). Three AP-PCR profiles were identified among these isolates. Three of these isolates had the same DNA bending pattern, and they were isolated from patients who were at the same institution. Conclusions: These preliminary results indicate that C. difficile may be involved in some diarrhea cases in China. This study may aid in the understanding of the nature of endemic diarrhea in this country.
KW - China
KW - Clostridium difficile
KW - Diarrhea
UR - http://www.scopus.com/inward/record.url?scp=0031430799&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031430799&partnerID=8YFLogxK
U2 - 10.1016/S1201-9712(97)90087-4
DO - 10.1016/S1201-9712(97)90087-4
M3 - Article
AN - SCOPUS:0031430799
VL - 2
SP - 85
EP - 87
JO - International Journal of Infectious Diseases
JF - International Journal of Infectious Diseases
SN - 1201-9712
IS - 2
ER -