Polymerase chain reaction for detection of Borrelia coriaceae, putative agent of epizootic bovine abortion.

B. C. Zingg, Rance B Lefebvre

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The nucleotide sequence of a chromosomally encoded antigen-expressing gene of Borrelia coriaceae was determined and used as a target for the polymerase chain reaction (PCR). Two primer sets were designed specifying the amplification of 269- and 701-bp DNA fragments. Primer set I, producing the short amplicon, was tenfold more sensitive than primer set II. As little as 10 fg of purified B coriaceae DNA could consistently be detected. The PCR assays, containing controlled numbers of whole spirochetes, allowed detectable amplification of 2 to 10 organisms. An internal, nonradioactively labeled gene-specific probe verified specificity of the PCR amplicons. Neither primer set cross-reacted with other related spirochetes. This PCR assay was adapted and found suitable for identification of B coriaceae in biological samples, such as blood and thymus. Evidence for presence of B coriaceae in biological samples was not found in tissue samples obtained from experimentally infected cows and their fetuses. These data failed to establish a definite association between B coriaceae and epizootic bovine abortion.

Original languageEnglish (US)
Pages (from-to)1509-1515
Number of pages7
JournalAmerican Journal of Veterinary Research
Volume55
Issue number11
StatePublished - Nov 1994

Fingerprint

Borrelia coriaceae
Borrelia
abortion (animals)
polymerase chain reaction
Polymerase Chain Reaction
Spirochaetales
cattle
DNA
assays
sampling
Thymus Gland
Genes
fetus
Fetus
genes
antigens
Antigens
nucleotide sequences
cows
blood

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Polymerase chain reaction for detection of Borrelia coriaceae, putative agent of epizootic bovine abortion. / Zingg, B. C.; Lefebvre, Rance B.

In: American Journal of Veterinary Research, Vol. 55, No. 11, 11.1994, p. 1509-1515.

Research output: Contribution to journalArticle

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