Polymerase chain reaction analysis of distal vaginal specimens: A less invasive strategy for detection of Trichomonas vaginalis

R. Phillips Heine, Harold C. Wiesenfeld, Richard L Sweet, Steven S. Witkin

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We compared polymerase chain reaction (PCR) analysis of specimens obtained from the distal vagina with wet mount microscopy and culture of specimens from the posterior vaginal fornix. One or all three techniques revealed that 61 (20.3%) of 300 women tested were positive for Trichomonas vaginalis. PCR analysis of distal vagina/specimens detected 56 (91.8%) of 61 infections, while wet mount microscopy and culture detected 49 (80.3%) of 61 infections. Results of this study may impact the approach to testing for T. vaginalis by eliminating the requirement of a vaginal speculum examination. The distal vagina is an appropriate testing site for T. vaginalis by PCR analysis.

Original languageEnglish (US)
Pages (from-to)985-987
Number of pages3
JournalClinical Infectious Diseases
Volume24
Issue number5
StatePublished - 1997
Externally publishedYes

Fingerprint

Trichomonas vaginalis
Vagina
Polymerase Chain Reaction
Microscopy
Gynecological Examination
Infection
Surgical Instruments

ASJC Scopus subject areas

  • Immunology

Cite this

Polymerase chain reaction analysis of distal vaginal specimens : A less invasive strategy for detection of Trichomonas vaginalis. / Heine, R. Phillips; Wiesenfeld, Harold C.; Sweet, Richard L; Witkin, Steven S.

In: Clinical Infectious Diseases, Vol. 24, No. 5, 1997, p. 985-987.

Research output: Contribution to journalArticle

@article{a58cfa2155674203a83b6fe708b2ef7c,
title = "Polymerase chain reaction analysis of distal vaginal specimens: A less invasive strategy for detection of Trichomonas vaginalis",
abstract = "We compared polymerase chain reaction (PCR) analysis of specimens obtained from the distal vagina with wet mount microscopy and culture of specimens from the posterior vaginal fornix. One or all three techniques revealed that 61 (20.3{\%}) of 300 women tested were positive for Trichomonas vaginalis. PCR analysis of distal vagina/specimens detected 56 (91.8{\%}) of 61 infections, while wet mount microscopy and culture detected 49 (80.3{\%}) of 61 infections. Results of this study may impact the approach to testing for T. vaginalis by eliminating the requirement of a vaginal speculum examination. The distal vagina is an appropriate testing site for T. vaginalis by PCR analysis.",
author = "Heine, {R. Phillips} and Wiesenfeld, {Harold C.} and Sweet, {Richard L} and Witkin, {Steven S.}",
year = "1997",
language = "English (US)",
volume = "24",
pages = "985--987",
journal = "Clinical Infectious Diseases",
issn = "1058-4838",
publisher = "Oxford University Press",
number = "5",

}

TY - JOUR

T1 - Polymerase chain reaction analysis of distal vaginal specimens

T2 - A less invasive strategy for detection of Trichomonas vaginalis

AU - Heine, R. Phillips

AU - Wiesenfeld, Harold C.

AU - Sweet, Richard L

AU - Witkin, Steven S.

PY - 1997

Y1 - 1997

N2 - We compared polymerase chain reaction (PCR) analysis of specimens obtained from the distal vagina with wet mount microscopy and culture of specimens from the posterior vaginal fornix. One or all three techniques revealed that 61 (20.3%) of 300 women tested were positive for Trichomonas vaginalis. PCR analysis of distal vagina/specimens detected 56 (91.8%) of 61 infections, while wet mount microscopy and culture detected 49 (80.3%) of 61 infections. Results of this study may impact the approach to testing for T. vaginalis by eliminating the requirement of a vaginal speculum examination. The distal vagina is an appropriate testing site for T. vaginalis by PCR analysis.

AB - We compared polymerase chain reaction (PCR) analysis of specimens obtained from the distal vagina with wet mount microscopy and culture of specimens from the posterior vaginal fornix. One or all three techniques revealed that 61 (20.3%) of 300 women tested were positive for Trichomonas vaginalis. PCR analysis of distal vagina/specimens detected 56 (91.8%) of 61 infections, while wet mount microscopy and culture detected 49 (80.3%) of 61 infections. Results of this study may impact the approach to testing for T. vaginalis by eliminating the requirement of a vaginal speculum examination. The distal vagina is an appropriate testing site for T. vaginalis by PCR analysis.

UR - http://www.scopus.com/inward/record.url?scp=0030938933&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030938933&partnerID=8YFLogxK

M3 - Article

C2 - 9142807

AN - SCOPUS:0030938933

VL - 24

SP - 985

EP - 987

JO - Clinical Infectious Diseases

JF - Clinical Infectious Diseases

SN - 1058-4838

IS - 5

ER -