Polylysine enhances cationic liposome-mediated transfection of the hepatoblastoma cell line Hep G2

Karl D. Mack, Rosemary L. Walzem, Karin Lehmann-Bruinsma, Jerry S Powell, Jerome B. Zeldis

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Plasmid DNA condensed by polylysine enhanced cationic-liposome-mediated transfection of Hep G2 cells. The luciferase expression plasmid pCMVL was complexed with the polycation poly-L-lysine and mixed with liposomes that contained a 1:1 molar ratio of the cationic lipid 1,2-dioleoyloxy-3- trimethyl-ammoniumpropane, with the neutral phospholipid 1,2-di-(cis-9- octadecenoyl)-sn-glycero-3-phosphoethanolamine. Polylysine enhanced cationic- liposome-mediated transfection of the hepatoblastoma cell line Hep G2 9-fold compared with pCMVL complexed alone with liposomes. The ratio of cationic to anionic charge of the polylysine-pCMVL complexes, and the quantity of cationic liposomes, are important determinants for optimal transfection of Hep G2 cells.

Original languageEnglish (US)
Pages (from-to)217-220
Number of pages4
JournalBiotechnology and Applied Biochemistry
Volume23
Issue number3
StatePublished - 1996

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology

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