Abstract
Plasmid DNA condensed by polylysine enhanced cationic-liposome-mediated transfection of Hep G2 cells. The luciferase expression plasmid pCMVL was complexed with the polycation poly-L-lysine and mixed with liposomes that contained a 1:1 molar ratio of the cationic lipid 1,2-dioleoyloxy-3- trimethyl-ammoniumpropane, with the neutral phospholipid 1,2-di-(cis-9- octadecenoyl)-sn-glycero-3-phosphoethanolamine. Polylysine enhanced cationic- liposome-mediated transfection of the hepatoblastoma cell line Hep G2 9-fold compared with pCMVL complexed alone with liposomes. The ratio of cationic to anionic charge of the polylysine-pCMVL complexes, and the quantity of cationic liposomes, are important determinants for optimal transfection of Hep G2 cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 217-220 |
| Number of pages | 4 |
| Journal | Biotechnology and Applied Biochemistry |
| Volume | 23 |
| Issue number | 3 |
| State | Published - 1996 |
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biotechnology