Abstract
EM9 is a mutagen-sensitive CHO cell whose phenotype resembles that of normal CHO cells exposed to 3-aminobenzamide, an inhibitor of poly(ADP-ribose) synthesis. This phenotype suggested that EM9 might be defective in poly(ADP-ribose) metabolism, but we now cannot find any abnormality in the synthesis or in the degradation of poly(ADP-ribose) in permeabilized EM9 cells. Thus the effects of 3-aminobenzamide on wild-type cells may be due to the inhibition of processes other than poly(ADP-ribose) synthesis. 3-Aminobenzamide enhances the cytotoxicity of EMS toward EM9 and control cells to the same degree.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 213-220 |
| Number of pages | 8 |
| Journal | Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis |
| Volume | 128 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1984 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Molecular Biology
- Health, Toxicology and Mutagenesis
- Medicine(all)
Cite this
Poly(ADP-ribose) metabolism appears normal in EM9, a mutagen-sensitive mutant of CHO cells. / Ikejima, Miyoko; Bohannon, Dian; Michael Gill, D.; Thompson, Larry H.
In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 128, No. 2, 1984, p. 213-220.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Poly(ADP-ribose) metabolism appears normal in EM9, a mutagen-sensitive mutant of CHO cells
AU - Ikejima, Miyoko
AU - Bohannon, Dian
AU - Michael Gill, D.
AU - Thompson, Larry H.
PY - 1984
Y1 - 1984
N2 - EM9 is a mutagen-sensitive CHO cell whose phenotype resembles that of normal CHO cells exposed to 3-aminobenzamide, an inhibitor of poly(ADP-ribose) synthesis. This phenotype suggested that EM9 might be defective in poly(ADP-ribose) metabolism, but we now cannot find any abnormality in the synthesis or in the degradation of poly(ADP-ribose) in permeabilized EM9 cells. Thus the effects of 3-aminobenzamide on wild-type cells may be due to the inhibition of processes other than poly(ADP-ribose) synthesis. 3-Aminobenzamide enhances the cytotoxicity of EMS toward EM9 and control cells to the same degree.
AB - EM9 is a mutagen-sensitive CHO cell whose phenotype resembles that of normal CHO cells exposed to 3-aminobenzamide, an inhibitor of poly(ADP-ribose) synthesis. This phenotype suggested that EM9 might be defective in poly(ADP-ribose) metabolism, but we now cannot find any abnormality in the synthesis or in the degradation of poly(ADP-ribose) in permeabilized EM9 cells. Thus the effects of 3-aminobenzamide on wild-type cells may be due to the inhibition of processes other than poly(ADP-ribose) synthesis. 3-Aminobenzamide enhances the cytotoxicity of EMS toward EM9 and control cells to the same degree.
UR - http://www.scopus.com/inward/record.url?scp=0021149732&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021149732&partnerID=8YFLogxK
U2 - 10.1016/0027-5107(84)90109-X
DO - 10.1016/0027-5107(84)90109-X
M3 - Article
C2 - 6472314
AN - SCOPUS:0021149732
VL - 128
SP - 213
EP - 220
JO - Mutation Research
JF - Mutation Research
SN - 0027-5107
IS - 2
ER -