Polarizing intestinal epithelial cells electrically through Ror2

Lin Cao, Colin D. McCaig, Roderick H. Scott, Siwei Zhao, Gillian Milne, Hans Clevers, Min Zhao, Jin Pu

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The apicobasal polarity of enterocytes determines where the brush border membrane (apical membrane) will form, but how this apical membrane faces the lumen is not well understood. The electrical signal across the epithelium could serve as a coordinating cue, orienting and polarizing enterocytes. Here, we show that applying a physiological electric field to intestinal epithelial cells, to mimic the natural electric field created by the transepithelial potential difference, polarized phosphorylation of the actin-binding protein ezrin, increased expression of intestinal alkaline phosphatase (ALPI, a differentiation marker) and remodeled the actin cytoskeleton selectively on the cathode side. In addition, an applied electric field also activated ERK1/2 and LKB1 (also known as STK11), key molecules in apical membrane formation. Disruption of the tyrosine protein kinase transmembrane receptor Ror2 suppressed activation of ERK1/2 and LKB1 significantly, and subsequently inhibited apical membrane formation in enterocytes. Our findings indicate that the endogenous electric field created by the transepithelial potential difference might act as an essential coordinating signal for apical membrane formation at a tissue level, through activation of LKB1 mediated by Ror2-ERK signaling.

Original languageEnglish (US)
Pages (from-to)3233-3239
Number of pages7
JournalJournal of Cell Science
Volume127
Issue number15
DOIs
StatePublished - 2014

Fingerprint

Epithelial Cells
Membranes
Enterocytes
Microfilament Proteins
Differentiation Antigens
Receptor Protein-Tyrosine Kinases
Microvilli
Actin Cytoskeleton
Cues
Alkaline Phosphatase
Electrodes
Epithelium
Phosphorylation

Keywords

  • Cell polarization
  • Electric field
  • Intestinal epithelial cells
  • Ror2
  • Transepithelial potential difference

ASJC Scopus subject areas

  • Cell Biology

Cite this

Cao, L., McCaig, C. D., Scott, R. H., Zhao, S., Milne, G., Clevers, H., ... Pu, J. (2014). Polarizing intestinal epithelial cells electrically through Ror2. Journal of Cell Science, 127(15), 3233-3239. https://doi.org/10.1242/jcs.146357

Polarizing intestinal epithelial cells electrically through Ror2. / Cao, Lin; McCaig, Colin D.; Scott, Roderick H.; Zhao, Siwei; Milne, Gillian; Clevers, Hans; Zhao, Min; Pu, Jin.

In: Journal of Cell Science, Vol. 127, No. 15, 2014, p. 3233-3239.

Research output: Contribution to journalArticle

Cao, L, McCaig, CD, Scott, RH, Zhao, S, Milne, G, Clevers, H, Zhao, M & Pu, J 2014, 'Polarizing intestinal epithelial cells electrically through Ror2', Journal of Cell Science, vol. 127, no. 15, pp. 3233-3239. https://doi.org/10.1242/jcs.146357
Cao L, McCaig CD, Scott RH, Zhao S, Milne G, Clevers H et al. Polarizing intestinal epithelial cells electrically through Ror2. Journal of Cell Science. 2014;127(15):3233-3239. https://doi.org/10.1242/jcs.146357
Cao, Lin ; McCaig, Colin D. ; Scott, Roderick H. ; Zhao, Siwei ; Milne, Gillian ; Clevers, Hans ; Zhao, Min ; Pu, Jin. / Polarizing intestinal epithelial cells electrically through Ror2. In: Journal of Cell Science. 2014 ; Vol. 127, No. 15. pp. 3233-3239.
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