Polarized cultures of human airway epithelium from nasal scrapings and bronchial brushings

Nilceia Lopez-Souza, Pedro C. Avila, Jonathan Widdicombe

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Airway epithelial cultures are generally derived from tracheas postmortem or from surgical specimens of nasal polyps or turbinates. Scrapings of the mucosal surface have been little used as starting material for cultures because of their low yield of epithelial cells and their contamination with mucous secretions, blood, and underlying connective tissue. For the first time, we report that human airway epithelial cells obtained from nasal scrapings or bronchial brushings can be grown in culture to produce polarized cell sheets suitable for studies of vectorial transport.

Original languageEnglish (US)
Pages (from-to)266-269
Number of pages4
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume39
Issue number7
DOIs
StatePublished - Jul 2003

Keywords

  • Airway epithelium
  • Bronchial brushing
  • Cell culture
  • Nasal scraping

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

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