Abstract
Airway epithelial cultures are generally derived from tracheas postmortem or from surgical specimens of nasal polyps or turbinates. Scrapings of the mucosal surface have been little used as starting material for cultures because of their low yield of epithelial cells and their contamination with mucous secretions, blood, and underlying connective tissue. For the first time, we report that human airway epithelial cells obtained from nasal scrapings or bronchial brushings can be grown in culture to produce polarized cell sheets suitable for studies of vectorial transport.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 266-269 |
| Number of pages | 4 |
| Journal | In Vitro Cellular and Developmental Biology - Animal |
| Volume | 39 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jul 2003 |
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Keywords
- Airway epithelium
- Bronchial brushing
- Cell culture
- Nasal scraping
ASJC Scopus subject areas
- Developmental Biology
- Clinical Biochemistry
- Cell Biology
Cite this
Polarized cultures of human airway epithelium from nasal scrapings and bronchial brushings. / Lopez-Souza, Nilceia; Avila, Pedro C.; Widdicombe, Jonathan.
In: In Vitro Cellular and Developmental Biology - Animal, Vol. 39, No. 7, 07.2003, p. 266-269.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Polarized cultures of human airway epithelium from nasal scrapings and bronchial brushings
AU - Lopez-Souza, Nilceia
AU - Avila, Pedro C.
AU - Widdicombe, Jonathan
PY - 2003/7
Y1 - 2003/7
N2 - Airway epithelial cultures are generally derived from tracheas postmortem or from surgical specimens of nasal polyps or turbinates. Scrapings of the mucosal surface have been little used as starting material for cultures because of their low yield of epithelial cells and their contamination with mucous secretions, blood, and underlying connective tissue. For the first time, we report that human airway epithelial cells obtained from nasal scrapings or bronchial brushings can be grown in culture to produce polarized cell sheets suitable for studies of vectorial transport.
AB - Airway epithelial cultures are generally derived from tracheas postmortem or from surgical specimens of nasal polyps or turbinates. Scrapings of the mucosal surface have been little used as starting material for cultures because of their low yield of epithelial cells and their contamination with mucous secretions, blood, and underlying connective tissue. For the first time, we report that human airway epithelial cells obtained from nasal scrapings or bronchial brushings can be grown in culture to produce polarized cell sheets suitable for studies of vectorial transport.
KW - Airway epithelium
KW - Bronchial brushing
KW - Cell culture
KW - Nasal scraping
UR - http://www.scopus.com/inward/record.url?scp=1042300191&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1042300191&partnerID=8YFLogxK
U2 - 10.1290/1543-706X(2003)039<0266:PCOHAE>2.0.CO;2
DO - 10.1290/1543-706X(2003)039<0266:PCOHAE>2.0.CO;2
M3 - Article
C2 - 14531727
AN - SCOPUS:1042300191
VL - 39
SP - 266
EP - 269
JO - In Vitro Cellular and Developmental Biology - Plant
JF - In Vitro Cellular and Developmental Biology - Plant
SN - 1054-5476
IS - 7
ER -