POLθ-mediated end joining is restricted by RAD52 and BRCA2 until the onset of mitosis

Marta Llorens-Agost, Michael Ensminger, Hang Phuong Le, Anugrah Gawai, Jie Liu, Andrés Cruz-García, Sarita Bhetawal, Richard D. Wood, Wolf-Dietrich Heyer, Markus Löbrich

Research output: Contribution to journalArticlepeer-review

Abstract

BRCA2-mutant cells are defective in homologous recombination, making them vulnerable to the inactivation of other pathways for the repair of DNA double-strand breaks (DSBs). This concept can be clinically exploited but is currently limited due to insufficient knowledge about how DSBs are repaired in the absence of BRCA2. We show that DNA polymerase θ (POLθ)-mediated end joining (TMEJ) repairs DSBs arising during the S phase in BRCA2-deficient cells only after the onset of the ensuing mitosis. This process is regulated by RAD52, whose loss causes the premature usage of TMEJ and the formation of chromosomal fusions. Purified RAD52 and BRCA2 proteins both block the DNA polymerase function of POLθ, suggesting a mechanism explaining their synthetic lethal relationships. We propose that the delay of TMEJ until mitosis ensures the conversion of originally one-ended DSBs into two-ended DSBs. Mitotic chromatin condensation might further serve to juxtapose correct break ends and limit chromosomal fusions.

Original languageEnglish (US)
Pages (from-to)1095-1104
Number of pages10
JournalNature Cell Biology
Volume23
Issue number10
DOIs
StatePublished - Oct 2021

ASJC Scopus subject areas

  • Cell Biology

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