PML-like subnuclear bodies, containing XRCC1, juxtaposed to DNA replication-based single-strand breaks

Magdalena M. Kordon, Aleksander Szczurek, Krzysztof Berniak, Oskar Szelest, Kamil Solarczyk, Magdalena Tworzydło, Sebastian Wachsmann-Hogiu, Anne Vaahtokari, Christoph Cremer, Thoru Pederson, Jurek W. Dobrucki

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

DNA lesions induce recruitment and accumulation of various repair factors, resulting in formation of discrete nuclear foci. Using superresolution fluorescence microscopy as well as live cell and quantitative imaging, we demonstrate that X-ray repair cross-complementing protein 1 (XRCC1), a key factor in single-strand break and base excision repair, is recruited into nuclear bodies formed in response to replication-related single-strand breaks. Intriguingly, these bodies are assembled immediately in the vicinity of these breaks and never fully colocalize with replication foci. They are structurally organized, containing canonical promyelocytic leukemia (PML) nuclear body protein SP100 concentrated in a peripheral layer, and XRCC1 in the center. They also contain other factors, including PML, poly(ADP-ribose) polymerase 1 (PARP1), ligase IIIα, and origin recognition complex subunit 5. The breast cancer 1 and -2 C terminus domains of XRCC1 are essential for formation of these repair foci. These results reveal that XRCC1-contaning foci constitute newly recognized PML-like nuclear bodies that accrete and locally deliver essential factors for repair of single-strand DNA breaks in replication regions.-Kordon, M. M., Szczurek, A., Berniak, K., Szelest, O., Solarczyk, K., Tworzydło, M., Wachsmann-Hogiu, S., Vaahtokari, A., Cremer, C., Pederson, T., Dobrucki, J. W. PML-like subnuclear bodies, containing XRCC1, juxtaposed to DNA replication-based single-strand breaks.

Original languageEnglish (US)
Pages (from-to)2301-2313
Number of pages13
JournalFASEB journal : official publication of the Federation of American Societies for Experimental Biology
Volume33
Issue number2
DOIs
StatePublished - Feb 1 2019

Fingerprint

DNA Replication
Leukemia
Repair
DNA
Origin Recognition Complex
Single-Stranded DNA Breaks
Poly(ADP-ribose) Polymerases
Fluorescence microscopy
Ligases
Nuclear Proteins
Fluorescence Microscopy
DNA Repair
X-ray repair cross complementing protein 1
Breast Neoplasms
Imaging techniques
Proteins

Keywords

  • DNA damage
  • PML nuclear bodies
  • superresolution microscopy

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

PML-like subnuclear bodies, containing XRCC1, juxtaposed to DNA replication-based single-strand breaks. / Kordon, Magdalena M.; Szczurek, Aleksander; Berniak, Krzysztof; Szelest, Oskar; Solarczyk, Kamil; Tworzydło, Magdalena; Wachsmann-Hogiu, Sebastian; Vaahtokari, Anne; Cremer, Christoph; Pederson, Thoru; Dobrucki, Jurek W.

In: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 33, No. 2, 01.02.2019, p. 2301-2313.

Research output: Contribution to journalArticle

Kordon, MM, Szczurek, A, Berniak, K, Szelest, O, Solarczyk, K, Tworzydło, M, Wachsmann-Hogiu, S, Vaahtokari, A, Cremer, C, Pederson, T & Dobrucki, JW 2019, 'PML-like subnuclear bodies, containing XRCC1, juxtaposed to DNA replication-based single-strand breaks', FASEB journal : official publication of the Federation of American Societies for Experimental Biology, vol. 33, no. 2, pp. 2301-2313. https://doi.org/10.1096/fj.201801379R
Kordon, Magdalena M. ; Szczurek, Aleksander ; Berniak, Krzysztof ; Szelest, Oskar ; Solarczyk, Kamil ; Tworzydło, Magdalena ; Wachsmann-Hogiu, Sebastian ; Vaahtokari, Anne ; Cremer, Christoph ; Pederson, Thoru ; Dobrucki, Jurek W. / PML-like subnuclear bodies, containing XRCC1, juxtaposed to DNA replication-based single-strand breaks. In: FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2019 ; Vol. 33, No. 2. pp. 2301-2313.
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AU - Szelest, Oskar

AU - Solarczyk, Kamil

AU - Tworzydło, Magdalena

AU - Wachsmann-Hogiu, Sebastian

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AU - Dobrucki, Jurek W.

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AB - DNA lesions induce recruitment and accumulation of various repair factors, resulting in formation of discrete nuclear foci. Using superresolution fluorescence microscopy as well as live cell and quantitative imaging, we demonstrate that X-ray repair cross-complementing protein 1 (XRCC1), a key factor in single-strand break and base excision repair, is recruited into nuclear bodies formed in response to replication-related single-strand breaks. Intriguingly, these bodies are assembled immediately in the vicinity of these breaks and never fully colocalize with replication foci. They are structurally organized, containing canonical promyelocytic leukemia (PML) nuclear body protein SP100 concentrated in a peripheral layer, and XRCC1 in the center. They also contain other factors, including PML, poly(ADP-ribose) polymerase 1 (PARP1), ligase IIIα, and origin recognition complex subunit 5. The breast cancer 1 and -2 C terminus domains of XRCC1 are essential for formation of these repair foci. These results reveal that XRCC1-contaning foci constitute newly recognized PML-like nuclear bodies that accrete and locally deliver essential factors for repair of single-strand DNA breaks in replication regions.-Kordon, M. M., Szczurek, A., Berniak, K., Szelest, O., Solarczyk, K., Tworzydło, M., Wachsmann-Hogiu, S., Vaahtokari, A., Cremer, C., Pederson, T., Dobrucki, J. W. PML-like subnuclear bodies, containing XRCC1, juxtaposed to DNA replication-based single-strand breaks.

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