TY - JOUR
T1 - Ploidy-, gender-, and dose-dependent alteration of selected biomarkers in Clarias gariepinus treated with benzo[a]pyrene
AU - Karami, Ali
AU - Teh, Swee J
AU - Zakaria, Mohamad Pauzi
AU - Courtenay, Simon C.
PY - 2015/12/1
Y1 - 2015/12/1
N2 - Naturally-occurring and artificially-induced polyploids have been documented in various fish species but to date no comparison has been reported of the impacts of ploidy on fish biomarker responses to organic pollutants. This study describes effects of ploidy, gender, and dose on biliary fluorescent aromatic compound (FAC) concentrations, hepatic ethoxyresorufin- O-deethylase (EROD) and glutathione S-transferase (GST) activities in one of the most commonly cultured warm-water species, the African catfish Clarias gariepinus. Recently matured male and female diploid and triploid fish were intraperitoneally (i.p.) injected with 0, 5 or 25. mg/kg benzo[. a]pyrene (BaP) and liver and gallbladder were sampled 48. hr later. No significant differences were found between ploidies in bile concentrations of 7,8 dihydrodiolbenzo[. a]pyrene (7,8D BaP), 1-hydroxybenzo[. a]pyrene (1-OH BaP) or 3-hydroxybenzo[. a]pyrene (3-OH BaP). However, concentrations of the biliary FACs did differ between males and females at different dose of injection with generally higher concentrations in females at the low dose of BaP and higher concentrations in males at the higher BaP concentration. Hepatic EROD activity did not exhibit gender-dependent difference, whereas it was significantly higher in triploids than diploids. GST activities were not significantly influenced by any of the tested factors. This work advanced our understanding of the role of ploidy, gender, and dose in biotransformation of pollutants in fish.
AB - Naturally-occurring and artificially-induced polyploids have been documented in various fish species but to date no comparison has been reported of the impacts of ploidy on fish biomarker responses to organic pollutants. This study describes effects of ploidy, gender, and dose on biliary fluorescent aromatic compound (FAC) concentrations, hepatic ethoxyresorufin- O-deethylase (EROD) and glutathione S-transferase (GST) activities in one of the most commonly cultured warm-water species, the African catfish Clarias gariepinus. Recently matured male and female diploid and triploid fish were intraperitoneally (i.p.) injected with 0, 5 or 25. mg/kg benzo[. a]pyrene (BaP) and liver and gallbladder were sampled 48. hr later. No significant differences were found between ploidies in bile concentrations of 7,8 dihydrodiolbenzo[. a]pyrene (7,8D BaP), 1-hydroxybenzo[. a]pyrene (1-OH BaP) or 3-hydroxybenzo[. a]pyrene (3-OH BaP). However, concentrations of the biliary FACs did differ between males and females at different dose of injection with generally higher concentrations in females at the low dose of BaP and higher concentrations in males at the higher BaP concentration. Hepatic EROD activity did not exhibit gender-dependent difference, whereas it was significantly higher in triploids than diploids. GST activities were not significantly influenced by any of the tested factors. This work advanced our understanding of the role of ploidy, gender, and dose in biotransformation of pollutants in fish.
KW - Diploid
KW - Female
KW - Fish biomarkers
KW - Male
KW - Triploid
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U2 - 10.1016/j.jes.2015.05.009
DO - 10.1016/j.jes.2015.05.009
M3 - Article
C2 - 26702972
AN - SCOPUS:84952975783
VL - 38
SP - 95
EP - 102
JO - Journal of Environmental Sciences
JF - Journal of Environmental Sciences
SN - 1001-0742
ER -