Platelet-rich fibrin constructs elute higher concentrations of transforming growth factor-β1 and increase tendon cell proliferation over time when compared to blood clots

A comparative in vitro analysis

Lance C Visser, Steven P. Arnoczky, Oscar Caballero, Monika Egerbacher

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Objective: To compare the concentration of a representative growth factor (transforming growth factor-beta [TGF-β]1) eluted from a platelet-rich fibrin matrix (PRFMatrix), a platelet-rich fibrin membrane (PRFMembrane), and a whole blood clot (BC) over time, and to compare the mitogenic effect of the eluents from each construct. Study Design: In vitro study. Sample Population: PRFMatrix, PRFMembrane, and BC (n=4/construct/time point). Methods: Each construct was placed in tissue culture wells containing media for 7 days. The media was collected and replenished on days 1, 3, 5, and 7 and the concentration of eluted TGF-β1 was measured by enzyme-linked immunosorbent assay. Canine tendon cells were subjected to additional aliquots of the conditioned media and the amount of cell proliferation compared. Results: The media from both PRFM (PRFMatrix and PRFMembrane) constructs contained significantly more (P≤.026) TGF-β1 at days 1 and 3 and produced a significant increase (P≤.044) in cell proliferation at all time points compared with the BC. The PRFMembrane media contained significantly more (P≤.05) TGF-β1 at days 1 and 3 and produced a significant increase (P≤.002) in cell proliferation at all time points compared with the PRFMatrix. Conclusions: Both PRFM constructs are comprised of a dense fibrin scaffold that contains increased concentrations of TGF-β1 and are capable of increasing tendon cell proliferation over time when compared with a BC. Clinical Relevance: The sustained increase in growth factor availability in PRFM constructs may be beneficial in the healing of biologically compromised tissues.

Original languageEnglish (US)
Pages (from-to)811-817
Number of pages7
JournalVeterinary Surgery
Volume39
Issue number7
DOIs
StatePublished - Oct 2010
Externally publishedYes

Fingerprint

transforming growth factors
fibrin
Transforming Growth Factors
tendons
Fibrin
Tendons
cell proliferation
Thrombosis
Blood Platelets
transforming growth factor beta 1
Cell Proliferation
blood
Transforming Growth Factor beta
Membranes
growth factors
Intercellular Signaling Peptides and Proteins
In Vitro Techniques
Conditioned Culture Medium
in vitro studies
tissue culture

ASJC Scopus subject areas

  • veterinary(all)

Cite this

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title = "Platelet-rich fibrin constructs elute higher concentrations of transforming growth factor-β1 and increase tendon cell proliferation over time when compared to blood clots: A comparative in vitro analysis",
abstract = "Objective: To compare the concentration of a representative growth factor (transforming growth factor-beta [TGF-β]1) eluted from a platelet-rich fibrin matrix (PRFMatrix), a platelet-rich fibrin membrane (PRFMembrane), and a whole blood clot (BC) over time, and to compare the mitogenic effect of the eluents from each construct. Study Design: In vitro study. Sample Population: PRFMatrix, PRFMembrane, and BC (n=4/construct/time point). Methods: Each construct was placed in tissue culture wells containing media for 7 days. The media was collected and replenished on days 1, 3, 5, and 7 and the concentration of eluted TGF-β1 was measured by enzyme-linked immunosorbent assay. Canine tendon cells were subjected to additional aliquots of the conditioned media and the amount of cell proliferation compared. Results: The media from both PRFM (PRFMatrix and PRFMembrane) constructs contained significantly more (P≤.026) TGF-β1 at days 1 and 3 and produced a significant increase (P≤.044) in cell proliferation at all time points compared with the BC. The PRFMembrane media contained significantly more (P≤.05) TGF-β1 at days 1 and 3 and produced a significant increase (P≤.002) in cell proliferation at all time points compared with the PRFMatrix. Conclusions: Both PRFM constructs are comprised of a dense fibrin scaffold that contains increased concentrations of TGF-β1 and are capable of increasing tendon cell proliferation over time when compared with a BC. Clinical Relevance: The sustained increase in growth factor availability in PRFM constructs may be beneficial in the healing of biologically compromised tissues.",
author = "Visser, {Lance C} and Arnoczky, {Steven P.} and Oscar Caballero and Monika Egerbacher",
year = "2010",
month = "10",
doi = "10.1111/j.1532-950X.2010.00739.x",
language = "English (US)",
volume = "39",
pages = "811--817",
journal = "Veterinary Surgery",
issn = "0161-3499",
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number = "7",

}

TY - JOUR

T1 - Platelet-rich fibrin constructs elute higher concentrations of transforming growth factor-β1 and increase tendon cell proliferation over time when compared to blood clots

T2 - A comparative in vitro analysis

AU - Visser, Lance C

AU - Arnoczky, Steven P.

AU - Caballero, Oscar

AU - Egerbacher, Monika

PY - 2010/10

Y1 - 2010/10

N2 - Objective: To compare the concentration of a representative growth factor (transforming growth factor-beta [TGF-β]1) eluted from a platelet-rich fibrin matrix (PRFMatrix), a platelet-rich fibrin membrane (PRFMembrane), and a whole blood clot (BC) over time, and to compare the mitogenic effect of the eluents from each construct. Study Design: In vitro study. Sample Population: PRFMatrix, PRFMembrane, and BC (n=4/construct/time point). Methods: Each construct was placed in tissue culture wells containing media for 7 days. The media was collected and replenished on days 1, 3, 5, and 7 and the concentration of eluted TGF-β1 was measured by enzyme-linked immunosorbent assay. Canine tendon cells were subjected to additional aliquots of the conditioned media and the amount of cell proliferation compared. Results: The media from both PRFM (PRFMatrix and PRFMembrane) constructs contained significantly more (P≤.026) TGF-β1 at days 1 and 3 and produced a significant increase (P≤.044) in cell proliferation at all time points compared with the BC. The PRFMembrane media contained significantly more (P≤.05) TGF-β1 at days 1 and 3 and produced a significant increase (P≤.002) in cell proliferation at all time points compared with the PRFMatrix. Conclusions: Both PRFM constructs are comprised of a dense fibrin scaffold that contains increased concentrations of TGF-β1 and are capable of increasing tendon cell proliferation over time when compared with a BC. Clinical Relevance: The sustained increase in growth factor availability in PRFM constructs may be beneficial in the healing of biologically compromised tissues.

AB - Objective: To compare the concentration of a representative growth factor (transforming growth factor-beta [TGF-β]1) eluted from a platelet-rich fibrin matrix (PRFMatrix), a platelet-rich fibrin membrane (PRFMembrane), and a whole blood clot (BC) over time, and to compare the mitogenic effect of the eluents from each construct. Study Design: In vitro study. Sample Population: PRFMatrix, PRFMembrane, and BC (n=4/construct/time point). Methods: Each construct was placed in tissue culture wells containing media for 7 days. The media was collected and replenished on days 1, 3, 5, and 7 and the concentration of eluted TGF-β1 was measured by enzyme-linked immunosorbent assay. Canine tendon cells were subjected to additional aliquots of the conditioned media and the amount of cell proliferation compared. Results: The media from both PRFM (PRFMatrix and PRFMembrane) constructs contained significantly more (P≤.026) TGF-β1 at days 1 and 3 and produced a significant increase (P≤.044) in cell proliferation at all time points compared with the BC. The PRFMembrane media contained significantly more (P≤.05) TGF-β1 at days 1 and 3 and produced a significant increase (P≤.002) in cell proliferation at all time points compared with the PRFMatrix. Conclusions: Both PRFM constructs are comprised of a dense fibrin scaffold that contains increased concentrations of TGF-β1 and are capable of increasing tendon cell proliferation over time when compared with a BC. Clinical Relevance: The sustained increase in growth factor availability in PRFM constructs may be beneficial in the healing of biologically compromised tissues.

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U2 - 10.1111/j.1532-950X.2010.00739.x

DO - 10.1111/j.1532-950X.2010.00739.x

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SP - 811

EP - 817

JO - Veterinary Surgery

JF - Veterinary Surgery

SN - 0161-3499

IS - 7

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