Platelet function abnormalities in qualified whole-blood donors

Effects of medication and recent food intake

T. G. Paglieroni, K. Janatpour, R. Gosselin, V. Crocker, Denis M Dwyre, M. R. MacKenzie, P. V. Holland, E. C. Larkin

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background and Objectives: Platelet function abnormalities have been reported in blood donors who have not consumed aspirin. Our objective was to identify factors other than aspirin that may contribute to impaired platelet function in qualified volunteer blood donors. Materials and Methods: Blood samples were obtained from 24 donors following routine blood donation. Donors completed a study questionnaire that included questions about recent food consumption, medication and medical history. Platelet activation was measured using monoclonal antibodies and flow cytometry. CD62P expression and PAC-1 binding on platelets were used as indicators of platelet activation. Platelet function was measured on a platelet function analyser (PFA-100®) using both collagen/epinephrine (cEPI) and collagen/ADP (cADP) cartridges. Results: Fifty-four per cent of donors (13 of 24) had normal platelet function. Thirty-eight per cent (nine of 24) had prolonged cEPI closure times, of whom four (17%) had no cEPI closure (> 300 seconds). No closure was associated with aspirin use (two donors) or chocolate consumption (two donors) before donation. Two donors (8%) had either a shortened cEPI or cADP closure time. Conclusions: Platelet dysfunction in qualified blood donors is underestimated. Platelet function screening can identify donors with diet-related platelet dysfunction or with poor recollection of aspirin use.

Original languageEnglish (US)
Pages (from-to)48-53
Number of pages6
JournalVox Sanguinis
Volume86
Issue number1
DOIs
StatePublished - Jan 2004

Fingerprint

Blood Donors
Blood Platelets
Eating
Tissue Donors
Collagen
Epinephrine
Aspirin
Platelet Activation
Adenosine Diphosphate
Volunteers
Flow Cytometry
Monoclonal Antibodies
Diet
Food

Keywords

  • 'Aspirin-like' defect
  • PFA-100®
  • Platelet activation
  • Platelet function

ASJC Scopus subject areas

  • Hematology

Cite this

Paglieroni, T. G., Janatpour, K., Gosselin, R., Crocker, V., Dwyre, D. M., MacKenzie, M. R., ... Larkin, E. C. (2004). Platelet function abnormalities in qualified whole-blood donors: Effects of medication and recent food intake. Vox Sanguinis, 86(1), 48-53. https://doi.org/10.1111/j.0042-9007.2004.00384.x

Platelet function abnormalities in qualified whole-blood donors : Effects of medication and recent food intake. / Paglieroni, T. G.; Janatpour, K.; Gosselin, R.; Crocker, V.; Dwyre, Denis M; MacKenzie, M. R.; Holland, P. V.; Larkin, E. C.

In: Vox Sanguinis, Vol. 86, No. 1, 01.2004, p. 48-53.

Research output: Contribution to journalArticle

Paglieroni, TG, Janatpour, K, Gosselin, R, Crocker, V, Dwyre, DM, MacKenzie, MR, Holland, PV & Larkin, EC 2004, 'Platelet function abnormalities in qualified whole-blood donors: Effects of medication and recent food intake', Vox Sanguinis, vol. 86, no. 1, pp. 48-53. https://doi.org/10.1111/j.0042-9007.2004.00384.x
Paglieroni, T. G. ; Janatpour, K. ; Gosselin, R. ; Crocker, V. ; Dwyre, Denis M ; MacKenzie, M. R. ; Holland, P. V. ; Larkin, E. C. / Platelet function abnormalities in qualified whole-blood donors : Effects of medication and recent food intake. In: Vox Sanguinis. 2004 ; Vol. 86, No. 1. pp. 48-53.
@article{b18d2f452e764a779a5fc64157469a49,
title = "Platelet function abnormalities in qualified whole-blood donors: Effects of medication and recent food intake",
abstract = "Background and Objectives: Platelet function abnormalities have been reported in blood donors who have not consumed aspirin. Our objective was to identify factors other than aspirin that may contribute to impaired platelet function in qualified volunteer blood donors. Materials and Methods: Blood samples were obtained from 24 donors following routine blood donation. Donors completed a study questionnaire that included questions about recent food consumption, medication and medical history. Platelet activation was measured using monoclonal antibodies and flow cytometry. CD62P expression and PAC-1 binding on platelets were used as indicators of platelet activation. Platelet function was measured on a platelet function analyser (PFA-100{\circledR}) using both collagen/epinephrine (cEPI) and collagen/ADP (cADP) cartridges. Results: Fifty-four per cent of donors (13 of 24) had normal platelet function. Thirty-eight per cent (nine of 24) had prolonged cEPI closure times, of whom four (17{\%}) had no cEPI closure (> 300 seconds). No closure was associated with aspirin use (two donors) or chocolate consumption (two donors) before donation. Two donors (8{\%}) had either a shortened cEPI or cADP closure time. Conclusions: Platelet dysfunction in qualified blood donors is underestimated. Platelet function screening can identify donors with diet-related platelet dysfunction or with poor recollection of aspirin use.",
keywords = "'Aspirin-like' defect, PFA-100{\circledR}, Platelet activation, Platelet function",
author = "Paglieroni, {T. G.} and K. Janatpour and R. Gosselin and V. Crocker and Dwyre, {Denis M} and MacKenzie, {M. R.} and Holland, {P. V.} and Larkin, {E. C.}",
year = "2004",
month = "1",
doi = "10.1111/j.0042-9007.2004.00384.x",
language = "English (US)",
volume = "86",
pages = "48--53",
journal = "Vox Sanguinis",
issn = "0042-9007",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Platelet function abnormalities in qualified whole-blood donors

T2 - Effects of medication and recent food intake

AU - Paglieroni, T. G.

AU - Janatpour, K.

AU - Gosselin, R.

AU - Crocker, V.

AU - Dwyre, Denis M

AU - MacKenzie, M. R.

AU - Holland, P. V.

AU - Larkin, E. C.

PY - 2004/1

Y1 - 2004/1

N2 - Background and Objectives: Platelet function abnormalities have been reported in blood donors who have not consumed aspirin. Our objective was to identify factors other than aspirin that may contribute to impaired platelet function in qualified volunteer blood donors. Materials and Methods: Blood samples were obtained from 24 donors following routine blood donation. Donors completed a study questionnaire that included questions about recent food consumption, medication and medical history. Platelet activation was measured using monoclonal antibodies and flow cytometry. CD62P expression and PAC-1 binding on platelets were used as indicators of platelet activation. Platelet function was measured on a platelet function analyser (PFA-100®) using both collagen/epinephrine (cEPI) and collagen/ADP (cADP) cartridges. Results: Fifty-four per cent of donors (13 of 24) had normal platelet function. Thirty-eight per cent (nine of 24) had prolonged cEPI closure times, of whom four (17%) had no cEPI closure (> 300 seconds). No closure was associated with aspirin use (two donors) or chocolate consumption (two donors) before donation. Two donors (8%) had either a shortened cEPI or cADP closure time. Conclusions: Platelet dysfunction in qualified blood donors is underestimated. Platelet function screening can identify donors with diet-related platelet dysfunction or with poor recollection of aspirin use.

AB - Background and Objectives: Platelet function abnormalities have been reported in blood donors who have not consumed aspirin. Our objective was to identify factors other than aspirin that may contribute to impaired platelet function in qualified volunteer blood donors. Materials and Methods: Blood samples were obtained from 24 donors following routine blood donation. Donors completed a study questionnaire that included questions about recent food consumption, medication and medical history. Platelet activation was measured using monoclonal antibodies and flow cytometry. CD62P expression and PAC-1 binding on platelets were used as indicators of platelet activation. Platelet function was measured on a platelet function analyser (PFA-100®) using both collagen/epinephrine (cEPI) and collagen/ADP (cADP) cartridges. Results: Fifty-four per cent of donors (13 of 24) had normal platelet function. Thirty-eight per cent (nine of 24) had prolonged cEPI closure times, of whom four (17%) had no cEPI closure (> 300 seconds). No closure was associated with aspirin use (two donors) or chocolate consumption (two donors) before donation. Two donors (8%) had either a shortened cEPI or cADP closure time. Conclusions: Platelet dysfunction in qualified blood donors is underestimated. Platelet function screening can identify donors with diet-related platelet dysfunction or with poor recollection of aspirin use.

KW - 'Aspirin-like' defect

KW - PFA-100®

KW - Platelet activation

KW - Platelet function

UR - http://www.scopus.com/inward/record.url?scp=1042288339&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1042288339&partnerID=8YFLogxK

U2 - 10.1111/j.0042-9007.2004.00384.x

DO - 10.1111/j.0042-9007.2004.00384.x

M3 - Article

VL - 86

SP - 48

EP - 53

JO - Vox Sanguinis

JF - Vox Sanguinis

SN - 0042-9007

IS - 1

ER -