Plasmonic nanostructures for bioanalytical applications of SERS

Mehmet Kahraman, Sebastian Wachsmann-Hogiu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations


Surface-enhanced Raman scattering (SERS) is a potential analytical technique for the detection and identification of chemicals and biological molecules and structures in the close vicinity of metallic nanostructures. We present a novel method to fabricate tunable plasmonic nanostructures and perform a comprehensive structural and optical characterization of the structures. Spherical latex particles are uniformly deposited on glass slides and used as templates to obtain nanovoid structures on polydimethylsiloxane surfaces. The diameter and depth of the nanovoids are controlled by the size of the latex particles. The nanovoids are coated with a thin Ag layer for fabrication of uniform plasmonic nanostructures. Structural characterization of the surfaces is performed by scanning electron microscopy (SEM) and atomic force microscopy (AFM). Optical properties of these plasmonic nanostructures are evaluated via UV/Vis spectroscopy, and SERS. The sample preparation step is the key point to obtain strong and reproducible SERS spectra from the biological structures. When the colloidal suspension is used as a SERS substrate for the protein detection, the electrostatic interaction of the proteins with the nanoparticles is described by the nature of their charge status, which influences the aggregation properties such as the size and shape of the aggregates, which is critical for the SERS experiment. However, when the solid SERS substrates are fabricated, SERS signal of the proteins that are background free and independent of the protein charge. Pros and cons of using plasmonic nano colloids and nanostructures as SERS substrate will be discussed for label-free detection of proteins using SERS.

Original languageEnglish (US)
Title of host publicationPlasmonics in Biology and Medicine XIII
ISBN (Electronic)9781628419580
StatePublished - 2016
EventPlasmonics in Biology and Medicine XIII - San Francisco, United States
Duration: Feb 15 2016Feb 16 2016


OtherPlasmonics in Biology and Medicine XIII
Country/TerritoryUnited States
CitySan Francisco


  • 3D nanostructures
  • nanovoid
  • plasmonic
  • protein
  • SERS

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging


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