Plasminogen is present in the basal layer of the epidermis

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Abstract

This study was undertaken to determine whether plasminogen was present within the epidermis. Cryostat secretions of normal human skin were incubated with either whole rabbit antihuman plasminogen serum, the IgG fraction thereof, or the immune-specific IgG fraction thereof, purified by affinity chromatography on plasminogen-Sepharose. Standard indirect immunofluorescent techniques using rhodamine-conjugated goat antirabbit IgG were subsequently employed. Fluorescence was localized to the basal layer of the epidermis. Nonimmune serum and immune serum depleted of its specific antiplasminogen IgG by prior affinity chromatography yielded negative results, demonstrating the specificity of staining for plasminogen. Our findings indicate that plasminogen is present within the epidermis, localized on the peripheral intracytoplasmic area of basal keratinocytes. This finding supports the hypothesis that a proteolytic event mediated by plasminogen activator with the subsequent generation of plasmin may be involved in normal keratinocyte physiology.

Original languageEnglish (US)
Pages (from-to)297-299
Number of pages3
JournalJournal of Investigative Dermatology
Volume80
Issue number4
StatePublished - 1983

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Plasminogen
Epidermis
Immunoglobulin G
Affinity chromatography
Keratinocytes
Affinity Chromatography
Rhodamines
Cryostats
Plasminogen Activators
Fibrinolysin
Physiology
Serum
Goats
Sepharose
Immune Sera
Skin
Fluorescence
Staining and Labeling
Rabbits

ASJC Scopus subject areas

  • Dermatology

Cite this

Plasminogen is present in the basal layer of the epidermis. / Isseroff, Roslyn Rivkah; Rifkin, D. B.

In: Journal of Investigative Dermatology, Vol. 80, No. 4, 1983, p. 297-299.

Research output: Contribution to journalArticle

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