Plasma membrane isolation and fatty acid analysis of membrane lipids from murine lymphocytes

Dorothy A. Adams; Sara J. Freauff; Kent L Erickson

doi:10.1016/0003-2697(85)90110-1

Plasma membrane isolation and fatty acid analysis of membrane lipids from murine lymphocytes

Dorothy A. Adams, Sara J. Freauff, Kent L Erickson

Cell Biology and Human Anatomy

Research output: Contribution to journal › Article › peer-review

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Abstract

Plasma membranes have been isolated in high yield by a novel method from splenic lymphocytes of BALB/c mice. These membranes were purified in predominantly envelope form, essentially right-side out, by zinc stabilization and sucrose density gradient centrifugation. A simple, rapid method was used for derivatization and analysis of fatty acids in plasma membrane lipids.

Original language	English (US)
Pages (from-to)	228-232
Number of pages	5
Journal	Analytical Biochemistry
Volume	144
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(85)90110-1
State	Published - 1985

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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title = "Plasma membrane isolation and fatty acid analysis of membrane lipids from murine lymphocytes",

abstract = "Plasma membranes have been isolated in high yield by a novel method from splenic lymphocytes of BALB/c mice. These membranes were purified in predominantly envelope form, essentially right-side out, by zinc stabilization and sucrose density gradient centrifugation. A simple, rapid method was used for derivatization and analysis of fatty acids in plasma membrane lipids.",

author = "Adams, {Dorothy A.} and Freauff, {Sara J.} and Erickson, {Kent L}",

year = "1985",

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AU - Freauff, Sara J.

AU - Erickson, Kent L

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AB - Plasma membranes have been isolated in high yield by a novel method from splenic lymphocytes of BALB/c mice. These membranes were purified in predominantly envelope form, essentially right-side out, by zinc stabilization and sucrose density gradient centrifugation. A simple, rapid method was used for derivatization and analysis of fatty acids in plasma membrane lipids.

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JO - Analytical Biochemistry

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Plasma membrane isolation and fatty acid analysis of membrane lipids from murine lymphocytes

Abstract

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