Abstract
Plasma membranes have been isolated in high yield by a novel method from splenic lymphocytes of BALB/c mice. These membranes were purified in predominantly envelope form, essentially right-side out, by zinc stabilization and sucrose density gradient centrifugation. A simple, rapid method was used for derivatization and analysis of fatty acids in plasma membrane lipids.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 228-232 |
| Number of pages | 5 |
| Journal | Analytical Biochemistry |
| Volume | 144 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1985 |
Fingerprint
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Plasma membrane isolation and fatty acid analysis of membrane lipids from murine lymphocytes. / Adams, Dorothy A.; Freauff, Sara J.; Erickson, Kent L.
In: Analytical Biochemistry, Vol. 144, No. 1, 1985, p. 228-232.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Plasma membrane isolation and fatty acid analysis of membrane lipids from murine lymphocytes
AU - Adams, Dorothy A.
AU - Freauff, Sara J.
AU - Erickson, Kent L
PY - 1985
Y1 - 1985
N2 - Plasma membranes have been isolated in high yield by a novel method from splenic lymphocytes of BALB/c mice. These membranes were purified in predominantly envelope form, essentially right-side out, by zinc stabilization and sucrose density gradient centrifugation. A simple, rapid method was used for derivatization and analysis of fatty acids in plasma membrane lipids.
AB - Plasma membranes have been isolated in high yield by a novel method from splenic lymphocytes of BALB/c mice. These membranes were purified in predominantly envelope form, essentially right-side out, by zinc stabilization and sucrose density gradient centrifugation. A simple, rapid method was used for derivatization and analysis of fatty acids in plasma membrane lipids.
UR - http://www.scopus.com/inward/record.url?scp=0021907216&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021907216&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(85)90110-1
DO - 10.1016/0003-2697(85)90110-1
M3 - Article
VL - 144
SP - 228
EP - 232
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 1
ER -