Plasma membrane isolation and fatty acid analysis of membrane lipids from murine lymphocytes

Dorothy A. Adams, Sara J. Freauff, Kent L Erickson

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Plasma membranes have been isolated in high yield by a novel method from splenic lymphocytes of BALB/c mice. These membranes were purified in predominantly envelope form, essentially right-side out, by zinc stabilization and sucrose density gradient centrifugation. A simple, rapid method was used for derivatization and analysis of fatty acids in plasma membrane lipids.

Original languageEnglish (US)
Pages (from-to)228-232
Number of pages5
JournalAnalytical Biochemistry
Volume144
Issue number1
DOIs
StatePublished - 1985

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Lymphocytes
Cell membranes
Membrane Lipids
Fatty Acids
Cell Membrane
Density Gradient Centrifugation
Centrifugation
Sucrose
Zinc
Stabilization
Membranes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Plasma membrane isolation and fatty acid analysis of membrane lipids from murine lymphocytes. / Adams, Dorothy A.; Freauff, Sara J.; Erickson, Kent L.

In: Analytical Biochemistry, Vol. 144, No. 1, 1985, p. 228-232.

Research output: Contribution to journalArticle

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