Plasma levels of fibronectin bearing the alternatively spliced EIIIB segment are increased after major trauma

John H. Peters, Grace A. Loredo, Ge Chen, Richard Maunder, Theodore J. Hahn, Neil H. Willits, Richard O. Hynes

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Using Western-blot analysis and enzyme-linked immunosorbent assay (ELISA) of N-deglycosylated samples, we have observed that plasma levels of fibronectin (FN) bearing the alternatively spliced EIIIB segment (EIIIB+ FN) increase in patients after admission to the intensive-care unit (ICU) for acute major trauma. Although not increased at the first sampling ("0 hour"), taken within 24 hours of ICU admission, levels measured 24, 48, and 72 hours later were significantly increased compared with levels obtained in healthy controls. Furthermore, average concentrations at these latter time points were significantly increased relative to the 0-hour sampling. EIIIB+ FN levels then decreased in plasma samples taken 1 month after hospital discharge, such that no significant difference was found between ELISA-measured values at this time and 0 hour or control values. On the basis of comparisons with previous measurements in these samples, it is apparent that after acute major trauma, circulating levels of soluble EIIIB+ FN exhibit temporal changes that are qualitatively similar to those encountered for FN isoforms bearing the alternatively spliced EIIIA segment (EIIIA+ FN), yet different from those observed for the total pool of circulating FN. This is the first report of measurement of FN in clinical plasma samples with antibodies specific for the highly conserved EIIIB segment. Like EIIIA+ forms of FN, EIIIB+ FNs are recognized as soluble bloodborne markers for vascular tissue injury.

Original languageEnglish (US)
Pages (from-to)401-410
Number of pages10
JournalJournal of Laboratory and Clinical Medicine
Volume141
Issue number6
DOIs
StatePublished - Jun 1 2003
Externally publishedYes

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Bearings (structural)
Fibronectins
Plasmas
Wounds and Injuries
Intensive care units
Immunosorbents
Intensive Care Units
Assays
Enzyme-Linked Immunosorbent Assay
Sampling
Patient Admission
Vascular System Injuries
Enzymes
Protein Isoforms
Western Blotting

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

Plasma levels of fibronectin bearing the alternatively spliced EIIIB segment are increased after major trauma. / Peters, John H.; Loredo, Grace A.; Chen, Ge; Maunder, Richard; Hahn, Theodore J.; Willits, Neil H.; Hynes, Richard O.

In: Journal of Laboratory and Clinical Medicine, Vol. 141, No. 6, 01.06.2003, p. 401-410.

Research output: Contribution to journalArticle

Peters, John H. ; Loredo, Grace A. ; Chen, Ge ; Maunder, Richard ; Hahn, Theodore J. ; Willits, Neil H. ; Hynes, Richard O. / Plasma levels of fibronectin bearing the alternatively spliced EIIIB segment are increased after major trauma. In: Journal of Laboratory and Clinical Medicine. 2003 ; Vol. 141, No. 6. pp. 401-410.
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