Plasma bupivacaine concentrations following orbital injections in cats

Yael Shilo-Benjamini, Bruno H Pypendop, Georgina Newbold, Peter J Pascoe

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objective: To determine plasma bupivacaine concentrations after retrobulbar or peribulbar injection of bupivacaine in cats. Study design: Randomized, crossover, experimental trial with a 2 week washout period. Animals: Six adult healthy cats, aged 1-2 years, weighing 4.6 ± 0.7 kg. Methods: Cats were sedated by intramuscular injection of dexmedetomidine (36-56 μg kg-1) and were administered a retrobulbar injection of bupivacaine (0.75 mL, 0.05%; 3.75 mg) and iopamidol (0.25 mL), or a peribulbar injection of bupivacaine (1.5 mL, 0.5%; 7.5 mg), iopamidol (0.5 mL) and 0.9% saline (1 mL) via a dorsomedial approach. Blood (2 mL) was collected before and at 5, 10, 15, 22, 30, 45, 60, 120, 240 and 480 minutes after bupivacaine injection. Atipamezole was administered approximately 30 minutes after bupivacaine injection. Plasma bupivacaine and 3-hydroxy-bupivacaine concentrations were determined using liquid chromatography-mass spectrometry. Bupivacaine maximum plasma concentration (Cmax) and time to Cmax (Tmax) were determined from the data. Results: The bupivacaine median (range) Cmax and Tmax were 1.4 (0.9-2.5) μg mL-1 and 17 (4-60) minutes, and 1.7 (1.0-2.4) μg mL-1, and 28 (8-49) minutes, for retrobulbar and peribulbar injections, respectively. In both treatments the 3-hydroxy-bupivacaine peak concentration was 0.05-0.21 μg mL-1. Conclusions and clinical relevance: In healthy cats, at doses up to 2 mg kg-1, bupivacaine peak plasma concentrations were approximately half that reported to cause arrhythmias or convulsive electroencephalogram (EEG) activity in cats, and about one-sixth of that required to produce hypotension.

Original languageEnglish (US)
JournalVeterinary Anaesthesia and Analgesia
DOIs
StateAccepted/In press - 2016

Fingerprint

Bupivacaine
Cats
cats
injection
Injections
Iopamidol
dexmedetomidine
electroencephalography
hypotension
arrhythmia
intramuscular injection
liquid chromatography
experimental design
mass spectrometry
Dexmedetomidine
Intramuscular Injections
blood
dosage
Liquid Chromatography
Hypotension

Keywords

  • Bupivacaine
  • Cats
  • Peribulbar anesthesia
  • Plasma concentration
  • Retrobulbar anesthesia

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Plasma bupivacaine concentrations following orbital injections in cats. / Shilo-Benjamini, Yael; Pypendop, Bruno H; Newbold, Georgina; Pascoe, Peter J.

In: Veterinary Anaesthesia and Analgesia, 2016.

Research output: Contribution to journalArticle

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abstract = "Objective: To determine plasma bupivacaine concentrations after retrobulbar or peribulbar injection of bupivacaine in cats. Study design: Randomized, crossover, experimental trial with a 2 week washout period. Animals: Six adult healthy cats, aged 1-2 years, weighing 4.6 ± 0.7 kg. Methods: Cats were sedated by intramuscular injection of dexmedetomidine (36-56 μg kg-1) and were administered a retrobulbar injection of bupivacaine (0.75 mL, 0.05{\%}; 3.75 mg) and iopamidol (0.25 mL), or a peribulbar injection of bupivacaine (1.5 mL, 0.5{\%}; 7.5 mg), iopamidol (0.5 mL) and 0.9{\%} saline (1 mL) via a dorsomedial approach. Blood (2 mL) was collected before and at 5, 10, 15, 22, 30, 45, 60, 120, 240 and 480 minutes after bupivacaine injection. Atipamezole was administered approximately 30 minutes after bupivacaine injection. Plasma bupivacaine and 3-hydroxy-bupivacaine concentrations were determined using liquid chromatography-mass spectrometry. Bupivacaine maximum plasma concentration (Cmax) and time to Cmax (Tmax) were determined from the data. Results: The bupivacaine median (range) Cmax and Tmax were 1.4 (0.9-2.5) μg mL-1 and 17 (4-60) minutes, and 1.7 (1.0-2.4) μg mL-1, and 28 (8-49) minutes, for retrobulbar and peribulbar injections, respectively. In both treatments the 3-hydroxy-bupivacaine peak concentration was 0.05-0.21 μg mL-1. Conclusions and clinical relevance: In healthy cats, at doses up to 2 mg kg-1, bupivacaine peak plasma concentrations were approximately half that reported to cause arrhythmias or convulsive electroencephalogram (EEG) activity in cats, and about one-sixth of that required to produce hypotension.",
keywords = "Bupivacaine, Cats, Peribulbar anesthesia, Plasma concentration, Retrobulbar anesthesia",
author = "Yael Shilo-Benjamini and Pypendop, {Bruno H} and Georgina Newbold and Pascoe, {Peter J}",
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T1 - Plasma bupivacaine concentrations following orbital injections in cats

AU - Shilo-Benjamini, Yael

AU - Pypendop, Bruno H

AU - Newbold, Georgina

AU - Pascoe, Peter J

PY - 2016

Y1 - 2016

N2 - Objective: To determine plasma bupivacaine concentrations after retrobulbar or peribulbar injection of bupivacaine in cats. Study design: Randomized, crossover, experimental trial with a 2 week washout period. Animals: Six adult healthy cats, aged 1-2 years, weighing 4.6 ± 0.7 kg. Methods: Cats were sedated by intramuscular injection of dexmedetomidine (36-56 μg kg-1) and were administered a retrobulbar injection of bupivacaine (0.75 mL, 0.05%; 3.75 mg) and iopamidol (0.25 mL), or a peribulbar injection of bupivacaine (1.5 mL, 0.5%; 7.5 mg), iopamidol (0.5 mL) and 0.9% saline (1 mL) via a dorsomedial approach. Blood (2 mL) was collected before and at 5, 10, 15, 22, 30, 45, 60, 120, 240 and 480 minutes after bupivacaine injection. Atipamezole was administered approximately 30 minutes after bupivacaine injection. Plasma bupivacaine and 3-hydroxy-bupivacaine concentrations were determined using liquid chromatography-mass spectrometry. Bupivacaine maximum plasma concentration (Cmax) and time to Cmax (Tmax) were determined from the data. Results: The bupivacaine median (range) Cmax and Tmax were 1.4 (0.9-2.5) μg mL-1 and 17 (4-60) minutes, and 1.7 (1.0-2.4) μg mL-1, and 28 (8-49) minutes, for retrobulbar and peribulbar injections, respectively. In both treatments the 3-hydroxy-bupivacaine peak concentration was 0.05-0.21 μg mL-1. Conclusions and clinical relevance: In healthy cats, at doses up to 2 mg kg-1, bupivacaine peak plasma concentrations were approximately half that reported to cause arrhythmias or convulsive electroencephalogram (EEG) activity in cats, and about one-sixth of that required to produce hypotension.

AB - Objective: To determine plasma bupivacaine concentrations after retrobulbar or peribulbar injection of bupivacaine in cats. Study design: Randomized, crossover, experimental trial with a 2 week washout period. Animals: Six adult healthy cats, aged 1-2 years, weighing 4.6 ± 0.7 kg. Methods: Cats were sedated by intramuscular injection of dexmedetomidine (36-56 μg kg-1) and were administered a retrobulbar injection of bupivacaine (0.75 mL, 0.05%; 3.75 mg) and iopamidol (0.25 mL), or a peribulbar injection of bupivacaine (1.5 mL, 0.5%; 7.5 mg), iopamidol (0.5 mL) and 0.9% saline (1 mL) via a dorsomedial approach. Blood (2 mL) was collected before and at 5, 10, 15, 22, 30, 45, 60, 120, 240 and 480 minutes after bupivacaine injection. Atipamezole was administered approximately 30 minutes after bupivacaine injection. Plasma bupivacaine and 3-hydroxy-bupivacaine concentrations were determined using liquid chromatography-mass spectrometry. Bupivacaine maximum plasma concentration (Cmax) and time to Cmax (Tmax) were determined from the data. Results: The bupivacaine median (range) Cmax and Tmax were 1.4 (0.9-2.5) μg mL-1 and 17 (4-60) minutes, and 1.7 (1.0-2.4) μg mL-1, and 28 (8-49) minutes, for retrobulbar and peribulbar injections, respectively. In both treatments the 3-hydroxy-bupivacaine peak concentration was 0.05-0.21 μg mL-1. Conclusions and clinical relevance: In healthy cats, at doses up to 2 mg kg-1, bupivacaine peak plasma concentrations were approximately half that reported to cause arrhythmias or convulsive electroencephalogram (EEG) activity in cats, and about one-sixth of that required to produce hypotension.

KW - Bupivacaine

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KW - Peribulbar anesthesia

KW - Plasma concentration

KW - Retrobulbar anesthesia

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