Placental DNA methylation levels at CYP2E1 and IRS2 are associated with child outcome in a prospective autism study

Yihui Zhu, Charles E. Mordaunt, Dag H. Yasui, Ria Marathe, Rochelle L. Coulson, Keith W. Dunaway, Julia M. Jianu, Cheryl K. Walker, Sally Ozonoff, Irva Hertz-Picciotto, Rebecca J. Schmidt, Janine M. LaSalle

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


DNA methylation acts at the interface of genetic and environmental factors relevant for autism spectrum disorder (ASD). Placenta, normally discarded at birth, is a potentially rich source of DNA methylation patterns predictive of ASD in the child. Here, we performed whole methylome analyses of placentas from a prospective study MARBLES (Markers of Autism Risk in Babies-Learning Early Signs) of high-risk pregnancies. A total of 400 differentially methylated regions (DMRs) discriminated placentas stored from children later diagnosed with ASD compared to typically developing controls. These ASD DMRs were significantly enriched at promoters, mapped to 596 genes functionally enriched in neuronal development, and overlapped genetic ASD risk. ASD DMRs at CYP2E1 and IRS2 reached genome-wide significance, replicated by pyrosequencing and correlated with expression differences in brain. Methylation at CYP2E1 associated with both ASD diagnosis and genotype within the DMR. In contrast, methylation at IRS2 was unaffected by within DMR genotype but modified by preconceptional maternal prenatal vitamin use. This study therefore identified two potentially useful early epigenetic markers for ASD in placenta.

Original languageEnglish (US)
Pages (from-to)2659-2674
Number of pages16
JournalHuman molecular genetics
Issue number16
StatePublished - Aug 15 2019

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)


Dive into the research topics of 'Placental DNA methylation levels at CYP2E1 and IRS2 are associated with child outcome in a prospective autism study'. Together they form a unique fingerprint.

Cite this